04-736 | Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

04-736
100 µl  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-736
      Replaces05-736
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal
      Alternate Names
      • AKT1 kinase
      • Protein kinase B
      • RAC-alpha serine/threonine-protein kinase
      • rac protein kinase alpha
      • v-akt murine thymoma viral oncogene homolog 1
      Background InformationThe serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKBα or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3). Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Untreated and PDGF treated NIH-3T3 fibroblasts.
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationAnti-Akt1 Antibody, clone SK703 detects level of phospho-Akt1/PKBα (Ser473) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa
      EpitopePhosphorylated Ser473
      CloneSK703
      HostRabbit
      SpecificityRecognizes phosphorylated Akt1/PKBα, Mr 60 kDa. Cross-reactivity to phosphorylated Akt2 and Akt3 likely based on sequence homology.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and mouse. Predicted cross-reactivity with rat, bovine, chicken, and Xenopus based on immunogen sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • RAC-ALPHA
      • PKB
      • MGC99656
      • RAC-PK-alpha
      • C-AKT
      • AKT
      • EC 2.7.11.1
      Modifications
      • Phosphorylation
      UniProt Number
      Molecular Weight60kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Akt1/PKB (Ser473), clone SK703 - 2029640 2029640
      Anti-phospho-Akt1/PKB (Ser473), clone SK703 - 2073589 2073589
      Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NG1752247 NG1752247
      Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NG1800782 NG1800782
      Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NG1848906 NG1848906
      Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NG1861479 NG1861479
      Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NRG1685842 NRG1685842
      Anti-phospho-Akt1/PKBα (Ser473), -2795414 2795414
      Anti-phospho-Akt1/PKBα (Ser473), clone SK703 -2585815 2585815
      Anti-phospho-Akt1/PKBα (Ser473), clone SK703 -2623587 2623587

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies