05-736 | Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

05-736
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-736
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal
      References
      Product Information
      FormatPurified
      PresentationProtein A purified antibody in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
      Applications
      ApplicationDetect phospho-Akt1/PKBα (Ser473) using this Anti-Akt1 Antibody, clone SK703 validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa
      CloneSK703
      HostRabbit
      Specificityphosphorylated Akt1/PKBa
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • RAC-ALPHA
      • PKB
      • MGC99656
      • RAC-PK-alpha
      • C-AKT
      • AKT
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight60kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Akt1/PKB#945; (Ser473), clone SK703 (rabbit monoclonal IgG) - DAM1821085 DAM1821085
      Anti-phospho-Akt1/PKB#945; (Ser473), clone SK703 - JBC1916932 JBC1916932
      Anti-phospho-Akt1/PKBa (Ser473), clone SK703 (rabbit monoclonal IgG) - 2225233 2225233
      Anti-phospho-Akt1/PKBa (Ser473), clone SK703 - 2075949 2075949
      Anti-phospho-Akt1/PKBa (Ser473), clone SK703 - DAM1690644 DAM1690644
      Anti-phospho-Akt1/PKBa (Ser473), clone SK703 - DAM1718048 DAM1718048
      Anti-phospho-Akt1/PKBa (Ser473), clone SK703 - DAM1807792 DAM1807792
      Anti-phospho-Akt1/PKBa (Ser473), clone SK703 - JBC1377938 JBC1377938
      Anti-phospho-Akt1/PKBalpha; (Ser473), clone SK703 - 24657 24657
      Anti-phospho-Akt1/PKBalpha; (Ser473), clone SK703 - 32515 32515

      References

      Reference overviewPub Med ID
      RLIP76 regulates PI3K/Akt signaling and chemo-radiotherapy resistance in pancreatic cancer.
      Leake, K; Singhal, J; Nagaprashantha, LD; Awasthi, S; Singhal, SS
      PloS one  7  e34582  2012

      Show Abstract
      22509328 22509328
      Resveratrol helps recovery from fatty liver and protects against hepatocellular carcinoma induced by hepatitis B virus X protein in a mouse model.
      Lin, HC; Chen, YF; Hsu, WH; Yang, CW; Kao, CH; Tsai, TF
      Cancer prevention research (Philadelphia, Pa.)  5  952-62  2012

      Show Abstract
      22659145 22659145
      Calyculin A from Discodermia calyx is a dual action toxin that blocks calcium influx and inhibits protein Ser/Thr phosphatases.
      Holy, M; Brautigan, DL
      Toxins  4  940-54  2012

      Show Abstract
      23162706 23162706
      The ATM kinase signaling induced by the low-energy β-particles emitted by (33)P is essential for the suppression of chromosome aberrations and is greater than that induced by the energetic β-particles emitted by (32)P.
      White JS, Yue N, Hu J, Bakkenist CJ
      Mutation research  708  28-36. Epub 2011 Feb 16.  2011

      Show Abstract
      21315088 21315088
      Cereal and nonfat milk support muscle recovery following exercise.
      Lynne Kammer, Zhenping Ding, Bei Wang, Daiske Hara, Yi-Hung Liao, John L Ivy, Lynne Kammer, Zhenping Ding, Bei Wang, Daiske Hara, Yi-Hung Liao, John L Ivy
      Journal of the International Society of Sports Nutrition  6  11  2009

      Show Abstract Full Text Article
      19442266 19442266
      Inducible-NOS but not neuronal-NOS participate in the acute effect of TNF-alpha on hypothalamic insulin-dependent inhibition of food intake.
      Juliana C Moraes, Maria E Amaral, Paty K Picardi, Vivian C Calegari, Talita Romanatto, Marcela Bermúdez-Echeverry, Silvana Chiavegatto, Mario J Saad, Licio A Velloso
      FEBS letters  580  4625-31  2006

      Show Abstract
      16876161 16876161
      3-Phosphoinositide-dependent protein kinase 1 (PDK1) phosphorylates and activates the p70 S6 kinase in vivo and in vitro.
      Alessi, D R, et al.
      Curr. Biol., 8: 69-81 (1998)  1998

      9427642 9427642
      PDK1, one of the missing links in insulin signal transduction?
      Cohen, P, et al.
      FEBS Lett., 410: 3-10 (1997)  1997

      Show Abstract
      9247112 9247112
      3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase.
      Alessi, D R, et al.
      Curr. Biol., 7: 776-89 (1997)  1997

      9368760 9368760
      Specific binding of the Akt-1 protein kinase to phosphatidylinositol 3,4,5-trisphosphate without subsequent activation.
      James, SR; Downes, CP; Gigg, R; Grove, SJ; Holmes, AB; Alessi, DR
      The Biochemical journal  315 ( Pt 3)  709-13  1996

      Show Abstract
      8645147 8645147

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