09-294 | Anti-phospho Calmodulin (Tyr138) Antibody

09-294
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Ch, Eq, R, CaWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-294
      DescriptionAnti-phospho Calmodulin (Tyr138) Antibody
      Alternate Names
      • calmodulin 1
      • calmodulin 1 (phosphorylase kinase, delta)
      • phosphorylase kinase, delta subunit
      Background InformationCalmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.
      CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.
      References
      Product Information
      FormatAffinity Purified
      Control
      • EGF treated and untreated HeLa cell lysate.
      PresentationAffinity purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho Calmodulin (Tyr138) Antibody detects level of phospho Calmodulin (Tyr138) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to mouse phosphorylated Calmodulin at and around Tyr138.
      EpitopePhosphorylated Tyr138
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes phosphorylated Calmodulin at Tyr138.
      Species Reactivity
      • Human
      • Mouse
      • Chicken
      • Horse
      • Rat
      • Canine
      Species Reactivity NoteReacts with human and mouse. Predicted to react with chicken, horse, rat, and dog based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCalmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.
      Gene Symbol
      • CALM1
      • CALML2
      • CALML2
      • CALM3
      • CALM
      • CaM
      • CALM2
      • CAM2
      • CAMC
      • CAM3
      • DD132
      • CAMIII
      • PHKD
      • CAMI
      • CAMB
      • CAM
      • CAM1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

      SUBUNIT STRUCTURE: Interacts with MYO1C By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY.

      SUBCELLULAR LOCATION: Spindle. Note= Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.

      PTM: Ubiquitination results in a strongly decreased activity By similarity.

      Phosphorylation results in a decreased activity By similarity.

      MISCELLANEOUS: This protein has four functional calcium-binding sites.

      SEQUENCE SIMILARITIES: Belongs to the calmodulin family.

      Contains 4 EF-hand domains.

      Sequence caution The sequence CAA36839.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 1:125 dilution of this antibody detected phosphorylated Calmodulin at Tyr138 in EGF treated and untreated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies