04-334 | Anti-phospho-Cbl (Tyr774) Antibody, rabbit monoclonal

04-334
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IF, IP, WB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-334
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Cbl (Tyr774) Antibody, rabbit monoclonal
      Alternate Names
      • Cas-Br-M (murine) ecotropic retroviral transforming sequence
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE: Pervanadate-treated Jurkat cells.
      PresentationRabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
      Applications
      ApplicationThis Anti-phospho-Cbl (Tyr774) Antibody, rabbit is validated for use in FC, IF, IP, WB, IH(P) for the detection of phospho-Cbl (Tyr774).
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blot: 1:100 on lysates from pervanadate-treated Jurkat cells.
      Immunoprecipitation: 1:20.
      Flow Cytometry: 1:20. Permeablization of cells is required.
      Immunohistochemistry: 1:25 on paraffin-embedded sections of human cervical carcinoma.
      Immunofluorescence: 1:25. Paraformaldehyde fixation recommended.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the amino acid residues surrounding tyrosine 774 of human Cbl.
      HostRabbit
      SpecificityRecognizes Cbl when phosphorylated on tyrosine 774.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
      Gene Symbol
      • CBL
      • CBL2
      • RNF55
      • C-CBL
      Modifications
      • Phosphorylation
      Non-Reactive Species
      • Mouse
      • Rat
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P22681 # Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
      SIZE: 906 amino acids; 99647 Da
      SUBUNIT: Associates with NCK via its SH3 domain. The phosphorylated C-terminus interacts with CD2AP via its second SH3 domain. Binds to UBE2L3. Interacts with adapters SLA, SLA2 and with the phosphorylated C-terminus of SH2B2. Interacts with EGFR, SYK and ZAP70 via the highly conserved Cbl-N region. Also interacts with SORBS1 and INPPL1/SHIP2. Interacts with phosphorylated LAT2. May interact with CBLB (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm.
      DOMAIN: SwissProt: P22681 The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. & The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
      PTM: Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.DISEASE:SwissProt: P22681 # Can be converted to an oncogenic protein by deletions or mutations that disturb its ability to down-regulate RTKs.
      SIMILARITY: Contains 1 CBL N-terminal domain. & Contains 2 EF-hand-like domains. & Contains 1 RING-type zinc finger. & Contains 1 SH2 domain. & Contains 1 UBA domain.
      MISCELLANEOUS: This protein has one functional calcium-binding site.
      Molecular Weight120 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Cbl (Tyr774), rabbit monoclonal - 0611045574 0611045574
      Anti-phospho-Cbl (Tyr774), rabbit monoclonal - 2108472 2108472
      Anti-phospho-Cbl (Tyr774), rabbit monoclonal - DAM1767544 DAM1767544
      Anti-phospho-Cbl (Tyr774), rabbit monoclonal - JBC1371300 JBC1371300
      Anti-phospho-Cbl (Tyr774), rabbit monoclonal -2485667 2485667

      References

      Reference overviewPub Med ID
      APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes
      Liu, Jun, et al
      Mol Cell Biol, 22:3599-609 (2002)  2002

      11997497 11997497
      Fyn, Yes, and Syk phosphorylation sites in c-Cbl map to the same tyrosine residues that become phosphorylated in activated T cells
      Feshchenko, E A, et al
      J Biol Chem, 273:8323-31 (1998)  1998

      9525940 9525940

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies