04-281 | Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Catalogue Number04-281
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Thr669) Antibody, clone 5F10
      Alternate Names
      • ERBB
      • ERBB1
      • epidermal growth factor receptor
      Product Information
      • Includes PMA and pervanadate treated A431 cell lysate as a positive control
      Presentation100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
      ApplicationAnti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.
      SpecificityRecognizes EGFR when phosphorylated at Threonine 669.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      • Phosphorylation
      Purification MethodThiophilic adsoption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions 6 months at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-EGFR (Thr669), clone 5F10 (Mouse monoclonal IgG1) 3118673
      Anti-phospho-EGFR (Thr669), clone 5F10 (Mouse monoclonal IgG1) - 2195988 2195988
      Anti-phospho-EGFR (Thr669), clone 5F10 - 0191S0201 0191S0201
      Anti-phospho-EGFR (Thr669), clone 5F10 - 2029610 2029610
      Anti-phospho-EGFR (Thr669), clone 5F10 - 2058318 2058318
      Anti-phospho-EGFR (Thr669), clone 5F10 - DAM1484509 DAM1484509
      Anti-phospho-EGFR (Thr669), clone 5F10 - NG1895478 NG1895478


      Reference overviewPub Med ID
      Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
      Winograd-Katz, S E and Levitzki, A
      Oncogene, 25:7381-90 (2006)  2006

      16785992 16785992
      Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
      Heisermann, G J, et al
      J Biol Chem, 265:12820-7 (1990)  1990

      2115882 2115882
      Epidermal growth factor receptor threonine and serine residues phosphorylated in vivo
      Heisermann, G J and Gill, G N
      J Biol Chem, 263:13152-8 (1988)  1988

      3138233 3138233

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      Life Science Research > Antibodies and Assays > Primary Antibodies