04-286 | Anti-phospho-EGFR (Tyr1086) Antibody, clone 8B8

04-286
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-286
      Replaces04-340
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Tyr1086) Antibody, clone 8B8
      Alternate Names
      • ERBB
      • ERBB1
      References
      Product Information
      FormatPurified
      Control
      • Includes pervanadate treated A431 cell lysate as a positive control
      Applications
      ApplicationAnti-phospho-EGFR (Tyr1086) Antibody, clone 8B8 is an antibody against phospho-EGFR (Tyr1086) for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr 1086 in human EGFR.
      Clone8B8
      HostMouse
      SpecificityRecognizes phosphorylated EGFR on Tyrosine 1086.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Modifications
      • Phosphorylation
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-EGFR (Tyr1086), clone 8B8 - 2139975 2139975
      Anti-phospho-EGFR (Tyr1086), clone 8B8 - 0188S0101 0188S0101
      Anti-phospho-EGFR (Tyr1086), clone 8B8 - 2315608 2315608

      References

      Reference overviewApplicationPub Med ID
      Tumor-initiating cells of HER2-positive carcinoma cell lines express the highest oncoprotein levels and are sensitive to trastuzumab.
      Magnifico, Alessandra, et al.
      Clin. Cancer Res., 15: 2010-21 (2009)  2009

      Show Abstract
      Immunoblotting (Western)19276287 19276287
      Modulation of survival signaling pathways and persistence of the genotoxic stress as a basis for the synergistic interaction between the atypical retinoid ST1926 and the epidermal growth factor receptor inhibitor ZD1839
      Zanchi, Chiara, et al
      Cancer Res, 65:2364-72 (2005)  2005

      15781651 15781651
      Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor
      Hsuan, J J, et al
      Biochem J, 262:659-63 (1989)  1989

      2803273 2803273

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies