04-341 | Anti-phospho-EGFR (Tyr1173) Antibody, rabbit monoclonal

04-341
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Human OnlyIF, IH(P), IP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-341
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Tyr1173) Antibody, rabbit monoclonal
      Alternate Names
      • ERBB
      • ERBB1
      • epidermal growth factor receptor
      References
      Product Information
      FormatPurified
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-phospho-EGFR (Tyr1173) Antibody, Rabbit is an antibody against phospho-EGFR (Tyr1173) for use in IF, IH(P), IP & WB.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the amino acid residues including and surrounding tyrosine 1173 of the human EGF Receptor (EGFR).
      HostRabbit
      SpecificityRecognizes human EGFR phosphorylated on tyrosine residue 1173.
      IsotypeIgG
      Species Reactivity
      • Human Only
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight170 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-EGFR (Tyr1173), -2567091 2567091
      Anti-phospho-EGFR (Tyr1173), -2584038 2584038
      Anti-phospho-EGFR (Tyr1173), Rabbit Monoclonal (Rabbit monoclonal IgG) - DAM1442536 DAM1442536
      Anti-phospho-EGFR (Tyr1173), Rabbit Monoclonal - 0701050537 0701050537
      Anti-phospho-EGFR (Tyr1173), Rabbit Monoclonal - 1951909 1951909
      Anti-phospho-EGFR (Tyr1173), Rabbit Monoclonal - 2008624 2008624
      Anti-phospho-EGFR (Tyr1173), Rabbit Monoclonal - 2041562 2041562
      Anti-phospho-EGFR (Tyr1173), Rabbit Monoclonal - 2051314 2051314
      Anti-phospho-EGFR (Tyr1173), Rabbit Monoclonal - 2297144 2297144
      Anti-phospho-EGFR (Tyr1173), Rabbit Monoclonal - DAM1411352 DAM1411352

      References

      Reference overviewPub Med ID
      Requirement of Tyr-992 and Tyr-1173 in phosphorylation of the epidermal growth factor receptor by ionizing radiation and modulation by SHP2
      Sturla, Lisa-Marie, et al
      J Biol Chem, 280:14597-604 (2005)  2005

      15708852 15708852
      Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines
      Moro, Laura, et al
      J Biol Chem, 277:9405-14 (2002)  2002

      11756413 11756413

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies