05-922R | Anti-phospho-Estrogen Receptor alpha (Ser305) Antibody, clone 124.9.4, rabbit monoclonal

05-922R
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-922R
      DescriptionAnti-phospho-Estrogen Receptor alpha (Ser305) Antibody, clone 124.9.4, rabbit monoclonal
      Alternate Names
      • Estrogen receptor
      • ER-alpha
      • Estradiol receptor
      • Nuclear receptor subfamily 3 group A member 1
      Background InformationEstrogen Receptor α (ERα) is a nuclear hormone receptor. This steroid hormone receptor is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. ERα is a ligand-activated transcription factor and upon being bound by a ligand such as estrogen, a conformational change occurs that allows dimerization and binding to an estrogen response element. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Two ERs are known, ERα, classically known as ER, and ERβ. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ appears to be distinct from those regulating ERα.
      References
      Product Information
      FormatPurified
      Control
      • PKA phosphorylated and unphosphorylated recombinant ERα protein.
      PresentationPurified rabbit monoclonal IgG in buffer containing PBS with 0.05% sodium azide.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Estrogen Receptor alpha in ductal carcinoma.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Estrogen Receptor alpha phosphorylated at Ser305.
      EpitopePhosphorylated Ser305
      Clone124.9.4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThia antibody recognizes Estrogen Receptor alpha phosphorylated at Ser305.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySummary: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq].
      Gene Symbol
      • ESR1
      • ESR
      • NR3A1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.

      SUBUNIT STRUCTURE: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with FOXC2, MAP1S, SLC30A9, UBE1C and NCOA3 coactivator (by similarity). Interacts with EP300; the interaction is estrogen-dependent and enhanced by CITED1. Interacts with CITED1; the interaction is estrogen-dependent. Interacts with NCOA5 and NCOA6 coactivators. Interacts with NCOA7; the interaction is a ligand-inducible. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. Interacts with KIF18A and LDB1. Interacts with RLIM (via C-terminus). Interacts with MACROD1. Interacts with SH2D4A and PLCG. Interaction with SH2D4A blocks binding to PLCG and inhibits estrogen-induced cell proliferation. Interacts with DYNLL1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes. Interacts with NR2C1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with DYX1C1. Interacts with PRMT2. Interacts with PI3KR1 or PI3KR2, SRC and PTK2. Interacts with RBFOX2.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm

      DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

      POST-TRANSLATIONAL MODIFICATION: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. Glycosylated; contains N-acetylglucosamine, probably O-linked. Ubiquitinated. Deubiquitinated by OTUB1. Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.

      POLYMORPHISM: Genetic variations in ESR1 are correlated with bone mineral density (BMD). Low BMD is a risk factor for osteoporotic fracture. Osteoporosis is characterized by reduced bone mineral density, disrutption of bone microarchitecture, and the alteration of the amount and variety of non-collagenous proteins in bone. Osteoporotic bones are more at risk of fracture.

      SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
      Molecular Weight~66 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PKA phosphorylated and unphosphorylated recombinant ERα protein.

      Western Blot Analysis: 1µg/mL dilution of this antibody detected Estrogen Receptor alpha on 10 µg of PKA phosphorylated and unphosphorylated recombinant ERα protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      05-922R -2616202 2616202
      Anti-phospho-Estrogen Receptor alpha (Ser305), 2475043
      Anti-phospho-Estrogen Receptor alpha (Ser305), clone 124.9.4 - 2161186 2161186
      Anti-phospho-Estrogen Receptor alpha (Ser305), clone 124.9.4 - 2431970 2431970
      Anti-phospho-Estrogen Receptor alpha (Ser305), clone 124.9.4 - 1983699 1983699
      Anti-phospho-Estrogen Receptor alpha (Ser305), clone 124.9.4 - 2026410 2026410
      Anti-phospho-Estrogen Receptor alpha (Ser305), clone 124.9.4 - 2227173 2227173
      Anti-phospho-Estrogen Receptor alpha (Ser305), clone 124.9.4 - NRG1906475 NRG1906475
      Anti-phospho-Estrogen Receptor alpha (Ser305), clone 124.9.4 -2520500 2520500

      References

      Reference overviewPub Med ID
      Elevated expression of TANK-binding kinase 1 enhances tamoxifen resistance in breast cancer.
      Wei, C; Cao, Y; Yang, X; Zheng, Z; Guan, K; Wang, Q; Tai, Y; Zhang, Y; Ma, S; Cao, Y; Ge, X; Xu, C; Li, J; Yan, H; Ling, Y; Song, T; Zhu, L; Zhang, B; Xu, Q; Hu, C; Bian, XW; He, X; Zhong, H
      Proceedings of the National Academy of Sciences of the United States of America  111  E601-10  2014

      Show Abstract
      24449872 24449872

      Brochure

      Title
      New Products: Volume 3, 2012

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies