04-974 | Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal

04-974
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-974
      DescriptionAnti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal
      Alternate Names
      • FADK 1
      • PTK2 protein tyrosine kinase 2
      • Protein-tyrosine kinase 2
      • focal adhesion kinase 1
      Background InformationFocal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
      References
      Product Information
      FormatUnpurified
      Control
      • Human and rat brain lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, Rabbit detects level of phospho-FAK (Tyr397) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPhospho-specific peptide surrounding tyrosine 397 of human FAK.
      EpitopeTyr397
      CloneEP2160Y
      HostRabbit
      SpecificityThis antibody detects FAK phosphorylated at Tyr397.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EC 2.7.10.2
      • FADK
      • FAK
      • FAK1
      • pp125FAK
      Modifications
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

      SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1.

      SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions.

      TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.

      DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity.

      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.

      PTM: Phosphorylated on 6 tyrosine residues upon activation.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

      Contains 1 FERM domain.

      Contains 1 protein kinase domain.

      Molecular Weight~ 125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Human and rat brain cell lysates.

      Western Blot Analysis: 1:1,000-2,000 dilution of this antibody was used to detect FAK in human and rat brain cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal - 2343599 2343599
      Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal - 2384746 2384746
      Anti-phospho-FAK (Tyr397), -2548171 2548171
      Anti-phospho-FAK (Tyr397), clone EP2160Y 2459510
      Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal - 04-974 04-974
      Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal - 2199729 2199729
      Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal - 2208789 2208789
      Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal - 2361886 2361886
      Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal - DAM1703532 DAM1703532
      Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal - JBC1768440 JBC1768440

      References

      Reference overviewPub Med ID
      FAK inhibition abrogates the malignant phenotype in aggressive pediatric renal tumors.
      Megison, ML; Gillory, LA; Stewart, JE; Nabers, HC; Mrozcek-Musulman, E; Beierle, EA
      Molecular cancer research : MCR  12  514-26  2014

      Show Abstract
      24464916 24464916

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      Life Science Research > Antibodies and Assays > Primary Antibodies