07-758 | Anti-phospho-Flt-1 (Tyr1213) Antibody

07-758
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Gt, H, M, R, ShWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-758
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Flt-1 (Tyr1213) Antibody
      Alternate Names
      • Vascular endothelial growth factor receptor 1 (VEGFR-1)
      • Vascular permeability factor receptor
      • Tyrosine-protein kinase FRT
      • Fms-like tyrosine kinase
      References
      Product Information
      FormatAffinity Purified
      Control
      • Recombinant Flt-1 (Catalogue No. 14-562) phosphorylated by ATP
      PresentationImmunoaffinity purified rabbit IgG in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-Flt-1 (Tyr1213) Antibody detects level of phospho-Flt-1 (Tyr1213) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence (...VR[pY]VN...) in which [pY] corresponds to phospho-tyrosine at residue 1213 of human Flt-1. The immunizing sequence is identical in marmoset, has 14/15 identical amino acids in rat, goat, sheep, cow, and rhesus monkey and 13/15 identical amino acids in mouse.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes phospho-Flt-1 (Tyr1213).
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Goat
      • Human
      • Mouse
      • Rat
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryOncogene FLT belongs to the src gene family and is related to oncogene ROS (MIM 165020). Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The sequence structure of the FLT gene resembles that of the FMS gene (MIM 164770); hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.[supplied by OMIM]
      Gene Symbol
      • FLT1
      • VEGFR1
      • FLT
      • FRT
      • Flt-1
      • VEGFR-1
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17948 # Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
      SIZE: 1338 amino acids; 150769 Da
      SUBUNIT: Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1.
      SUBCELLULAR LOCATION: Isoform Flt1: Cell membrane; Single-pass type I membrane protein. & Isoform sFlt1: Secreted.
      TISSUE SPECIFICITY: Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
      SIMILARITY: SwissProt: P17948 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight~180 kDa (66 kDa for the fragment tested in QC)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on recombinant Flt-1 (Catalog # 14-562) phosphorylated upon incubation with ATP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Flt-1 (Tyr1213) (rabbit immunoaffinity purified IgG) - 2120126 2120126
      Anti-phospho-Flt-1 (Tyr1213) (rabbit immunoaffinity purified IgG) - 2387525 2387525
      Anti-phospho-Flt-1 (Tyr1213) (rabbit immunoaffinity purified IgG) - 2464581 2464581
      Anti-phospho-Flt-1 (Tyr1213) (rabbit immunoaffinity purified IgG) 2884433
      Anti-phospho-Flt-1 (Tyr1213) (rabbit immunoaffinity purified IgG) - 2194151 2194151
      Anti-phospho-Flt-1 (Tyr1213) (rabbit immunoaffinity purified IgG) - 2326379 2326379
      Anti-phospho-Flt-1 (Tyr1213) (rabbit immunoaffinity purified IgG) -2502964 2502964
      Anti-phospho-Flt-1 (Tyr1213) - 2982403 2982403
      Anti-phospho-Flt-1 (Tyr1213) - 30621 30621
      Anti-phospho-Flt-1 (Tyr1213) - DAM1535095 DAM1535095

      References

      Reference overviewApplicationPub Med ID
      Intrinsic bevacizumab resistance is associated with prolonged activation of autocrine VEGF signaling and hypoxia tolerance in colorectal cancer cells and can be overcome by nintedanib, a small molecule angiokinase inhibitor.
      Mésange, P; Poindessous, V; Sabbah, M; Escargueil, AE; de Gramont, A; Larsen, AK
      Oncotarget  5  4709-21  2014

      Show Abstract
      Immunohistochemistry25015210 25015210
      Vascular protection by angiotensin receptor antagonism involves differential VEGF expression in both hemispheres after experimental stroke.
      Guan, W; Somanath, PR; Kozak, A; Goc, A; El-Remessy, AB; Ergul, A; Johnson, MH; Alhusban, A; Soliman, S; Fagan, SC
      PloS one  6  e24551  2011

      Show Abstract
      21912702 21912702
      EGFR- and VEGF(R)-targeted small molecules show synergistic activity in colorectal cancer models refractory to combinations of monoclonal antibodies.
      Poindessous, V; Ouaret, D; El Ouadrani, K; Battistella, A; Mégalophonos, VF; Kamsu-Kom, N; Petitprez, A; Escargueil, AE; Boudou, P; Dumont, S; Cervera, P; Fléjou, JF; André, T; Tournigand, C; Chibaudel, B; de Gramont, A; Larsen, AK
      Clinical cancer research : an official journal of the American Association for Cancer Research  17  6522-30  2011

      Show Abstract
      21880790 21880790
      Vascular endothelial growth factor blockade rapidly elicits alternative proangiogenic pathways in neuroblastoma.
      Zaghloul, N; Hernandez, SL; Bae, JO; Huang, J; Fisher, JC; Lee, A; Kadenhe-Chiweshe, A; Kandel, JJ; Yamashiro, DJ
      International journal of oncology  34  401-7  2009

      Show Abstract
      Immunohistochemistry, Immunofluorescence19148474 19148474
      Therapeutic targeting of neuropilin-2 on colorectal carcinoma cells implanted in the murine liver.
      Gray, MJ; Van Buren, G; Dallas, NA; Xia, L; Wang, X; Yang, AD; Somcio, RJ; Lin, YG; Lim, S; Fan, F; Mangala, LS; Arumugam, T; Logsdon, CD; Lopez-Berestein, G; Sood, AK; Ellis, LM
      Journal of the National Cancer Institute  100  109-20  2008

      Show Abstract
      18182619 18182619
      Vasculogenesis and angiogenesis.
      Patan, Sybill
      Cancer Treat. Res., 117: 3-32 (2004)  2004

      15015550 15015550

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies