07-1414 | Anti-phospho-Glycogen Synthase (muscle) (Ser640) Antibody

07-1414
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ELISARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1414
      DescriptionAnti-phospho-Glycogen Synthase (muscle) (Ser640) Antibody
      Alternate Names
      • Starchsynthase, muscle
      Background InformationA glycosyltransferase enzyme that catalyses the reaction of UDP-glucose and (1,4-α-D-glucosyl)n to yield UDP and (1,4-α-D-glucosyl)n+1. In other words, this enzyme converts excess glucose residues one by one into a polymeric chain for storage as glycogen. The reaction is highly regulated by allosteric effectors such as glucose-6-phosphate, by phosphorylation reactions, and indirectly triggered by the hormone insulin.
      References
      Product Information
      FormatAffinity Purified
      Control
      • PDGF treated NIH/3T3 cell lysate
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.02 M Potassium phosphate, pH 7.2 with 0.15 M Sodium chloride and 0.01% sodium azide.
      Applications
      ApplicationThis Anti-phospho-Glycogen Synthase (muscle) (Ser640) Antibody is validated for use in WB, ELISA for the detection of phospho-Glycogen Synthase (muscle) (Ser640).
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesDirect ELISA: 1:30,000-1:150,000 dilution of a previous lot was used in direct ELISA when assayed against 0.1 µg of the immunogen peptide.

      Optimal working dilutions must be determined by the end user.

      Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
      Biological Information
      ImmunogenSynthetic phospho peptide from human muscle Glycogen synthase.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityGlycogen synthase (muscle), phosphoSer640. By Western blot the antibody recognizes the ~84 kDa phosphorylated protein. Reactivity to non-phosphorylated human Glycogen synthase is minimal (less than 0.2%) by ELISA.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlycogen is a high molecular mass polysaccharide that serves as a repository of glucose for use in times of metabolic need. Glycogen synthase (EC 2.4.1.11) catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages (Pederson et al., 2004 [PubMed 15282316]).[supplied by OMIM]
      Gene Symbol
      • GYS1
      • Glycogen
      • GYS
      • GSY
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
      CATALYTIC ACTIVITY: UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).
      ENZYME REGULATION: Allosteric activation by glucose-6-phosphate. Phosphorylation reduces the activity towards UDP-glucose. When in the non-phosphorylated state, glycogen synthase does not require glucose-6-phosphate as an allosteric activator; when phosphorylated it does (By similarity).
      PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
      DISEASE: Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also called muscle glycogen synthase deficiency. GSD0 is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
      SIMILARITY: Belongs to the mammalian/fungal glycogen synthase family.
      Molecular WeightAntibody recognizes the ~84 kDa phosphorylated protein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PDGF treated NIH/3T3 lysates.

      Western Blot Analysis:
      1:500-1:1,000 dilution of this lot detected phosphorylated Glycogen Synthase on 10 μg of PDGF treated NIH/3T3 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted aliquotes from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies