06-1370 | Anti-phospho-Histone H1 (Thr165) Antibody

06-1370
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1370
      DescriptionAnti-phospho-Histone H1 (Thr165) Antibody
      Alternate Names
      • Histone H1.2
      • Histone H1d
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein
      PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-phospho-Histone H1 (Thr165) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect phospho-Histone H1 (Thr165) also known as Histone H1.2, Histone H1d.
      Key Applications
      • Western Blotting
      • Dot Blot
      Application NotesDot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-phospho-Histone H1 (Thr165) (1:5,000 dilution).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Histone H1 phosphorylated at Thr165.
      EpitopePhosphorylated Thr165
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Histone H1 phosphorylated at Thr165.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Broad species crossreactivity due to conserved homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H1C
      • H1F2
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~33 kDa observed. A cross-reacting band is observed at ~110 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 on 10 µg of untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H1 (Thr165) - 2450753 2450753
      Anti-phospho-Histone H1 (Thr165) - 2467144 2467144
      Anti-phospho-Histone H1 (Thr165) - Q2027062 Q2027062
      Anti-phospho-Histone H1 (Thr165) -2796797 2796797
      Anti-phospho-Histone H1 (Thr165) -2812291 2812291

      References

      Reference overviewPub Med ID
      Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications.
      Terme, Jean-Michel, et al.
      FEBS Lett., (2014)  2014

      Show Abstract
      24873882 24873882

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies