05-636-I | Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

200 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, ChIP, IFMPurifiedMonoclonal Antibody
      Catalogue Number05-636-I
      DescriptionAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
      Alternate Names
      • Histone H2A.x
      • H2a/x
      • H2A.XS139p
      • H2AXS139p
      Background InformationThe histone H2A.X protein is a variant member of the H2A family of histones that is distinguished from other H2A histones by a unique carboxy-terminal sequence. This unique sequence is highly conserved throughout eukaryotic evolution and is rapidly phosphorylated by ATM or ATR at the fourth residue from the carboxy-terminus (Serine 139 in mammalian H2A.X) in response to DNA double-strand breaks (DSBs). Phosphorylation of H2A.X is important in the formation of a stable repair complex at the site of DNA damage.
      H2A.X phosphorylation is a very rapid response to DNA damage, occurring within as little as one minute after exposure to ionizing radiation. Phosphorylation of H2A.X occurs irrespective of the cause of the DNA DSBs and phospho-H2A.X has been observed in response to environmental stresses that result in DSBs as well as programmed cellular events, including DNA rearrangement and apoptosis.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 is a highly specific mouse monoclonal antibody, that targets Histone H2A.X & has been tested in western blotting, ICC, ChIP & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      • Immunofluorescence
      Application NotesImmunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected phospho-Histone H2A. X (Ser139) in HeLa and A431 cells.
      Previous lot has been demonstrated to work in Immunofluorescence and Chromatin Immunoprecipitation: See reference (Meier, Andreas et al., 2007)
      Western Blotting Analysis: 0.05-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
      Immunocytochemistry: 2 μg/mL of a previous lot of antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5μM staurosporine for 4-6 hours.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phospho-Histone H2A. X (Ser139).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H2AFX
      • H2AX
      • H2A.X
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho-Histone H2A. X (Ser139) in 200 µg in staurosporine treated HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H2A.X (Ser139), -2723666 2723666
      Anti-phospho-Histone H2A.X (Ser139), -2745891 2745891
      Anti-phospho-Histone H2A.X (Ser139), -2794963 2794963
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 2861355
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2363579 2363579
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2361863 2361863
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - Q2303828 Q2303828
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 -2535296 2535296
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 -2552645 2552645
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 -2583450 2583450


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      Life Science Research > Antibodies and Assays > Primary Antibodies