07-179 | Anti-phospho-Histone H2A/H4 (Ser1) Antibody

07-179
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-179
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H2A/H4 (Ser1) Antibody
      Alternate Names
      • Histone H2A/H4 ((phospho S1)
      References
      Product Information
      FormatSerum
      PresentationAntiserum containing 0.05% sodium azide before the addition of glycerol
      Applications
      ApplicationAnti-phospho-Histone H2A/H4 (Ser1) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Histone H2A/H4 (Ser1) also known as Histone H2A/H4 ((phospho S1) & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to amino acids 1-9 of human histone H2A (pSGRGKQGGK-C)
      HostRabbit
      SpecificityPhosphorylated Histones H2A and H4
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Phosphorylation
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular WeightH2A (16kDa) and H4 (10kDa)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on acid-extracted proteins from colcemid-arrested HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H2A/H4 (Ser1) 2474898
      Anti-phospho-Histone H2A/H4 (Ser1) - 20341 20341
      Anti-phospho-Histone H2A/H4 (Ser1) - DAM1484458 DAM1484458

      References

      Reference overviewApplicationPub Med ID
      Phosphorylation and arginine methylation mark histone H2A prior to deposition during Xenopus laevis development.
      Wang, WL; Anderson, LC; Nicklay, JJ; Chen, H; Gamble, MJ; Shabanowitz, J; Hunt, DF; Shechter, D
      Epigenetics & chromatin  7  22  2014

      Show Abstract
      Immunoprecipitation25302076 25302076
      Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
      Shechter, D; Nicklay, JJ; Chitta, RK; Shabanowitz, J; Hunt, DF; Allis, CD
      The Journal of biological chemistry  284  1064-74  2009

      Show Abstract
      18957438 18957438
      Phosphorylation of histone H2A by protein kinase C and identification of the phosphorylation site.
      Takeuchi, F, et al.
      J. Biochem., 111: 788-92 (1992)  1992

      Show Abstract
      1500420 1500420