07-191 | Anti-phospho-Histone H2B (Ser14) Antibody

200 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, ICC, IHC, WBRbSerumPolyclonal Antibody
      Catalogue Number07-191
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H2B (Ser14) Antibody
      Alternate Names
      • H2BS14P
      • Histone H2B (phospho S14)
      • H2B type 12
      • histone 3
      • H2bb
      • histone H2B
      • v
      Background InformationModulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression.
      Product Information
      • Acid extracted HL-60 cells treated with VP16
      PresentationRabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
      ApplicationUse Anti-phospho-Histone H2B (Ser14) Antibody (Rabbit Polyclonal Antibody) validated in ELISA, ICC, IHC, WB to detect phospho-Histone H2B (Ser14) also known as H2BS14P, Histone H2B (phospho S14).
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 6-16 of human histone H2B (C-SAPAPKKG[pS]KK).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes phospho-Histone H2B (Ser14), Mr 15 kDa.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Broad species cross-reactivity expected based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
      Gene Symbol
      • HIST3H2BB
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 126 amino acids; 13908 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.
      SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.
      Molecular Weight15 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on acid extracted HL-60 cells treated with VP16.

      Western Blot Analysis:
      1:1,000-1:10,000 dilutions of this lot detected phospho-Histone H2B in acid extracted HL-60 cells treated with 20μg/mL VP16 for 18 hours. A 1:250-1:2,000 dilution of a previous lot also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells.

      The optimal antibody dilution of this antibody must be empirically determined. Use of this antibody at dilutions which are too low, will result in non-specific detection of Histone H2B and other unknown proteins in western blots of acid extracts from untreated cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H2B (Ser14) 2476957
      Anti-phospho-Histone H2B (Ser14) - 20373 20373
      Anti-phospho-Histone H2B (Ser14) - 25994 25994
      Anti-phospho-Histone H2B (Ser14) - 27710 27710
      Anti-phospho-Histone H2B (Ser14) - 32600 32600
      Anti-phospho-Histone H2B (Ser14) - DAM1413942 DAM1413942
      Anti-phospho-Histone H2B (Ser14) Polyclonal Antibody 2903459
      Anti-phospho-Histone H2B (Ser14) Polyclonal Antibody 2930350


      Reference overviewApplicationPub Med ID
      Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin.
      Sims, JK; Wade, PA
      Molecular biology of the cell  22  3094-102  2011

      Show Abstract
      Western Blotting21737684 21737684
      A semisynthetic strategy to generate phosphorylated and acetylated histone H2B.
      Chiang, KP; Jensen, MS; McGinty, RK; Muir, TW
      Chembiochem : a European journal of chemical biology  10  2182-7  2009

      Show Abstract
      19623598 19623598
      Histone deimination as a response to inflammatory stimuli in neutrophils.
      Indira Neeli,Salar N Khan,Marko Radic
      Journal of immunology (Baltimore, Md. : 1950)  180  2008

      Show Abstract
      18209087 18209087
      Histone modifications associated with somatic hypermutation.
      Odegard, VH; Kim, ST; Anderson, SM; Shlomchik, MJ; Schatz, DG
      Immunity  23  101-10  2005

      Show Abstract
      16039583 16039583


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      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics


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      Life Science Research > Antibodies and Assays > Primary Antibodies