07-492 | Anti-phospho-Histone H3 (Thr11) Antibody

07-492
200 µL  
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      Overview

      Replacement Information
      Replacement InformationMABE468 is the recommended replacement for Discontinued catalog number 07-492

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, VrtDB, IF, WB, MplexRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-492
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H3 (Thr11) Antibody
      Alternate Names
      • H3T11P
      • Histone H3 (phospho T11)
      References
      Product Information
      FormatAffinity Purified
      Presentation0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationUse Anti-phospho-Histone H3 (Thr11) Antibody (Rabbit Polyclonal Antibody) validated in DB, IF, WB, Mplex to detect phospho-Histone H3 (Thr11) also known as H3T11P, Histone H3 (phospho T11).
      Key Applications
      • Dot Blot
      • Immunofluorescence
      • Western Blotting
      • Multiplexing
      Biological Information
      ImmunogenPeptide containing (KS[pT]GG), in which pT corresponds to phosphothreonine 11 of human histone H3.
      HostRabbit
      Specificityhistone H3 phosphorylated at threonine 11
      IsotypeIgG
      Species Reactivity
      • Human
      • Vertebrates
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3/t
      • H3t
      • MGC126888
      • H3/g
      • H3.4
      • H3T
      • MGC126886
      Modifications
      • Phosphorylation
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot in acid extracted proteins from colcemid treated HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H3 (Thr11) - 24577 24577

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes.
      Mursalimov, S; Permyakova, N; Deineko, E; Houben, A; Demidov, D
      Frontiers in plant science  6  846  2015

      Show Abstract
      26528310 26528310
      Ablation of leptin signaling disrupts the establishment, development, and maintenance of endometriosis-like lesions in a murine model.
      Styer, AK; Sullivan, BT; Puder, M; Arsenault, D; Petrozza, JC; Serikawa, T; Chang, S; Hasan, T; Gonzalez, RR; Rueda, BR
      Endocrinology  149  506-14  2008

      Show Abstract Full Text Article
      Immunohistochemistry, Immunohistochemistry (Paraffin)Mouse17962343 17962343
      Adult neurogenesis: a common strategy across diverse species.
      Sullivan, JM; Benton, JL; Sandeman, DC; Beltz, BS
      The Journal of comparative neurology  500  574-84  2007

      Show Abstract
      17120293 17120293

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      Life Science Research > Antibodies and Assays > Primary Antibodies