06-1340 Anti-phospho-IKK-epsilon (Ser172) Antibody

100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number06-1340
      DescriptionAnti-phospho-IKK-epsilon (Ser172) Antibody
      Alternate Names
      • Inhibitor of nuclear factor kappa-B kinase subunit epsilon
      • I-kappa-B kinase epsilon
      • IKK-E
      • IKK-epsilon
      • IkBKE
      • Inducible I kappa-B kinase
      • IKK-i
      Background InformationInhibitor of nuclear factor kappa-B kinase subunit epsilon (UniProt: Q14164; also known as EC:, I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, Inducible I kappa-B kinase, IKK-i) is encoded by the IKBKE (also known as IKKE, IKKI, KIAA0151) gene (Gene ID: 9641) in human. IKK-epsilon is a homodimeric serine/threonine kinase that is highly expressed in spleen, thymus, peripheral blood leukocytes, pancreas, and placenta. Its activity is induced by lipopolysaccharide and TNF-alpha. It plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B, and STAT signaling. IKK-epsilon can be either be auto-phosphorylated or phosphorylated by IKBKB/IKKB and phosphorylation at serine172 is enhanced by the interaction with ATP-dependent RNA helicase DDX3X. Its ATP-binding site resides in amino acids 15 to 23. IKK-epsilon is reported to phosphorylate inhibitors of NF-kappa-B leading to their ubiquitination and degradation. Following activation of viral RNA sensors, such as RIG-I-like receptors, it associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Two isoforms of IKK-epsilon have been described that are produced by alternative splicing.
      Product Information
      FormatAffinity Purified
      • CD3+CD28 treated and untreated Jurkat cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 30% glycerol.
      ApplicationThis Anti-phospho-IKK-epsilon (Ser172) Antibody is validated for use in WB for the detection of phospho-IKK-epsilon (Ser172).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenLinear peptide corresponding to human IKKepsilon phosphorylated at Ser172.
      EpitopePhosphorylated Ser172
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes IKK-epsilon phosphorylated at Ser172.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM].
      Gene Symbol
      • IKBKE
      • IKKE
      • IKKI
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.

      CATALYTIC ACTIVITY: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

      SUBUNIT STRUCTURE: May interact with MAVS/IPS1. Interacts with AZI2. Interacts with SIKE1. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between IKBKE and SIKE1. Interacts with TOPORS; induced by DNA damage. Interacts with CYLD.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus › PML body. Note: Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.

      TISSUE SPECIFICITY: Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.

      PTM: Autophosphorylated.

      Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

      Contains 1 protein kinase domain.

      SEQUENCE CAUTION: The sequence BAA09772.2 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~90 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in CD3+CD28 treated and untreated Jurkat cell lysates.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected IKK-epsilon on 10 µg of CD3+CD28 treated and untreated Jurkat cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-IKK-epsilon (Ser172) - 19838351983835
      Anti-phospho-IKK-epsilon (Ser172) - 20260282026028
      Anti-phospho-IKK-epsilon (Ser172) - 22193662219366
      Anti-phospho-IKK-epsilon (Ser172) - 25142042514204
      Anti-phospho-IKK-epsilon (Ser172) - NG1943277NG1943277
      Anti-phospho-IKK-epsilon (Ser172) - NRG1881974NRG1881974
      Anti-phospho-IKK-epsilon (Ser172) -25817882581788
      Anti-phospho-IKK-epsilon (Ser172) -27201212720121
      Anti-phospho-IKK-epsilon (Ser172) -27422552742255
      Anti-phospho-IKK-epsilon (Ser172) -27459372745937


      Reference overviewPub Med ID
      Measles virus suppresses RIG-I-like receptor activation in dendritic cells via DC-SIGN-mediated inhibition of PP1 phosphatases.
      Mesman, AW; Zijlstra-Willems, EM; Kaptein, TM; de Swart, RL; Davis, ME; Ludlow, M; Duprex, WP; Gack, MU; Gringhuis, SI; Geijtenbeek, TB
      Cell host & microbe  16  31-42  2014

      Show Abstract
      25011106 25011106


      Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      07-580 Anti-IKKε Antibody Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Primary Antibodies