04-467 | Anti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)RbPurifiedMonoclonal Antibody
      Catalogue Number04-467
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal
      Product Information
      Presentation100 μL of rabbit monoclonal IgG in 60% storage buffer (50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-LAT (Tyr191) using this Anti-phospho-LAT (Tyr191) Antibody, Rabbit validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenKLH-conjugated synthetic phosphor-peptide encompassing residues surrounding Tyrosine 191 of human LAT.
      SpecificityRecognizes a synthetic phosphor-peptide encompassing residues surrounding Tyrosine 191 of human LAT. This antibody will react only with LAT phosphorylated at Tyrosine 191.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • LAT
      • pp36
      • LAT1
      • p36-38
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43561 # Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
      SIZE: 262 amino acids; 27930 Da
      SUBUNIT: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB and SKAP2.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
      TISSUE SPECIFICITY: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
      PTM: Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. & Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
      MISCELLANEOUS: Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell- induced activation of PLC, maybe by inducing the dephosphorylation of LAT.
      Molecular Weight36kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-LAT (Tyr191) (Rabbit monoclonal IgG) - 2135059 2135059
      Anti-phospho-LAT (Tyr191) (Rabbit monoclonal IgG) - 2466254 2466254
      Anti-phospho-LAT (Tyr191) (Rabbit monoclonal IgG) - 2292292 2292292
      Anti-phospho-LAT (Tyr191) (Rabbit monoclonal IgG) - DAM1460234 DAM1460234
      Anti-phospho-LAT (Tyr191) - 2034672 2034672
      Anti-phospho-LAT (Tyr191) - 2049191 2049191
      Anti-phospho-LAT (Tyr191) - 2494619 2494619
      Anti-phospho-LAT (Tyr191) - DAM1514050 DAM1514050
      Anti-phospho-LAT (Tyr191) - NG1676060 NG1676060
      Anti-phospho-LAT (Tyr191), Rabbit Monoclonal - 0611046370 0611046370


      Reference overviewApplicationPub Med ID
      Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.
      Driessens, G; Zheng, Y; Locke, F; Cannon, JL; Gounari, F; Gajewski, TF
      Journal of immunology (Baltimore, Md. : 1950)  186  784-90  2011

      Show Abstract
      21149602 21149602
      Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells
      Weber, J R, et al
      J Exp Med, 187:1157-61 (1998)  1998

      9529333 9529333
      LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.
      Zhang, W, et al.
      Cell, 92: 83-92 (1998)  1998

      Show Abstract
      Immunoblotting (Western)9489702 9489702

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      Life Science Research > Antibodies and Assays > Primary Antibodies