06-1374 | Anti-phospho MRE11 (Thr636) Antibody

06-1374
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1374
      DescriptionAnti-phospho MRE11 (Thr636) Antibody
      Alternate Names
      • MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
      • meiotic recombination (S. cerevisiae) 11 homolog A
      • double-strand break repair protein MRE11A
      • Meiotic recombination 11 homolog A
      • Meiotic recombination 11 homolog 1
      • DNA recombination and repair protein
      • MRE11 homolog 1
      • endo/exonuclease Mre11
      • MRE11 homolog A
      • AT-like disease
      Background InformationMRE11 is is a nuclear protein involved the DNA damge repair and recombination. This protein is part of the MRN complex composed of three main components: Mre11, Rad50, and Nbs1/Xrs2. This protein complex is involved in signaling pathways that lead to both checkpoint activation and DNA repair. Mutations and defects in expression of this protein can lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Anisomycin untreated and treated HEK293 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho MRE11 (Thr636) Antibody is a rabbit polyclonal antibody for detection of phospho MRE11 (Thr636) also known as MRE11 meiotic recombination 11 homolog A (S. cerevisiae), has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human MRE11 phosphorylated at Thr636.
      EpitopePhosphorylated Thr636
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes MRE11 phosphorylated at Thr636.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq].
      Gene Symbol
      • MRE11A
      • MRE11
      • MRE11B
      • ATLD
      • HNGS1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

      COFACTOR: Manganese (By similarity).

      SUBUNIT STRUCTURE: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN (By similarity). Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo.

      SUBCELLULAR LOCATION: Nucleus (By similarity). Note: Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity).

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

      INVOLVEMENT IN DISEASE: Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.

      MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.

      SEQUENCE SIMILARITIES: Belongs to the MRE11/RAD32 family.
      Molecular Weight~85 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in anisomycin untreated and treated HEK293 cell lysates.

      Western Blot Analysis: 1 µg/mL of this antibody detected MRE11 on 10 µg of anisomycin untreated and treated HEK293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications