05-773 | Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19, rabbit monoclonal

05-773
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, HWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-773
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-MYPT1 (Thr850) Antibody, clone SA19, rabbit monoclonal
      References
      Product Information
      FormatPurified
      Applications
      ApplicationAnti-phospho-MYPT1 (Thr850) Antibody, clone SA19 is an antibody against phospho-MYPT1 (Thr850) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...RS[pT]GV..., in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.
      CloneSA19
      HostRabbit
      SpecificityMYPT1 phosphorylated on Thr850
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      Species Reactivity NotePredicted to cross react with mouse and rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
      Gene Symbol
      • PPP1R12A
      • MYPT1
      • MGC133042
      • MBS
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.
      SIZE: 1030 amino acids; 115281 Da
      SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
      PTM: Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: O14974 ## Contains 6 ANK repeats.
      Molecular Weightdoublet at 130kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho MYPT-1 (Thr850), clone SA19 - 2424701 2424701
      Anti-phospho-MYPT1 (Thr850), clone SA19 (rabbit monoclonal IgG) 2854611
      Anti-phospho-MYPT1 (Thr850), clone SA19 (rabbit monoclonal IgG) - NG1830453 NG1830453
      Anti-phospho-MYPT1 (Thr850), clone SA19 - 26820 26820

      References

      Reference overviewPub Med ID
      Sustained therapeutic hypercapnia attenuates pulmonary arterial Rho-kinase activity and ameliorates chronic hypoxic pulmonary hypertension in juvenile rats.
      Peng, G; Ivanovska, J; Kantores, C; Van Vliet, T; Engelberts, D; Kavanagh, BP; Enomoto, M; Belik, J; Jain, A; McNamara, PJ; Jankov, RP
      American journal of physiology. Heart and circulatory physiology  302  H2599-611  2012

      Show Abstract
      22505643 22505643
      The vitamin D receptor agonist elocalcitol inhibits IL-8-dependent benign prostatic hyperplasia stromal cell proliferation and inflammatory response by targeting the RhoA/Rho kinase and NF-kB pathways.
      Giuseppe Penna, Benedetta Fibbi, Susana Amuchastegui, Elisa Corsiero, Gilles Laverny, Enrico Silvestrini, Aravinda Chavalmane, Annamaria Morelli, Erica Sarchielli, Gabriella Barbara Vannelli, Mauro Gacci, Enrico Colli, Mario Maggi, Luciano Adorini, Giuseppe Penna, Benedetta Fibbi, Susana Amuchastegui, Elisa Corsiero, Gilles Laverny, Enrico Silvestrini, Aravinda Chavalmane, Annamaria Morelli, Erica Sarchielli, Gabriella Barbara Vannelli, Mauro Gacci, Enrico Colli, Mario Maggi, Luciano Adorini, Giuseppe Penna, Benedetta Fibbi, Susana Amuchastegui, Elisa Corsiero, Gilles Laverny, Enrico Silvestrini, Aravinda Chavalmane, Annamaria Morelli, Erica Sarchielli, Gabriella Barbara Vannelli, Mauro Gacci, Enrico Colli, Mario Maggi, Luciano Adorini
      The Prostate  69  480-93  2009

      Show Abstract
      19107880 19107880
      Myosin phosphatase: structure, regulation and function.
      Ito, Masaaki, et al.
      Mol. Cell. Biochem., 259: 197-209 (2004)  2004

      Show Abstract
      15124925 15124925
      Role of protein phosphatase type 1 in contractile functions: myosin phosphatase.
      Hartshorne, David J, et al.
      J. Biol. Chem., 279: 37211-4 (2004)  2004

      15136561 15136561
      Hypertension and RhoA/Rho-kinase signaling in the vasculature: highlights from the recent literature.
      Lee, Dexter L, et al.
      Hypertension, 44: 796-9 (2004)  2004

      Show Abstract
      15520302 15520302
      Smooth muscle contraction and relaxation.
      Webb, R Clinton
      Advances in physiology education, 27: 201-6 (2003)  2003

      Show Abstract
      14627618 14627618
      RhoA-mediated Ca2+ sensitization in erectile function.
      Wang, Hua, et al.
      J. Biol. Chem., 277: 30614-21 (2002)  2002

      Show Abstract
      12060659 12060659

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies