07-810 | Anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) Antibody

07-810
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IF, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-810
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) Antibody
      Alternate Names
      • c-Met
      • cmet
      • HGFR
      • RCCP2
      • met proto-oncogene (hepatocyte growth factor receptor)
      References
      Product Information
      FormatAffinity Purified
      Presentation100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
      Applications
      ApplicationAnti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) Antibody is an antibody against phospho-Met (Tyr1230/Tyr1234/Tyr1235) for use in IC, IF & WB.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunoblot Analysis: A 1:1000 dilution of this lot detected phospho-Met (Tyr1230/Tyr1234/Tyr1235) in RIPA lysate of HGF treated 293T cells.
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-c-Met (Tyr1230/Tyr1234/Tyr1235)
      HostRabbit
      SpecificityRecognizes Met phosphorylated on both Tyr1234/Tyr1235 and Tyr1230/Tyr1234/Tyr1235.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteRat has not been tested, but expected to react based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.
      Gene Symbol
      • MET
      • AUTS9
      • c-Met
      • RCCP2
      • HGFR
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08581 # Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity.
      SIZE: 1390 amino acids; 155527 Da
      SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
      SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain.
      Molecular Weight155 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) - 0604027376 0604027376
      Anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) - 0612047262 0612047262
      Anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) - 0701050529 0701050529
      Anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) - DAM1398266 DAM1398266
      Anti-phospho-c-Met (Tyr1230/Tyr1234/Tyr1235) 2477806
      Anti-phospho-c-Met (Tyr1230/Tyr1234/Tyr1235) (Affinity purified Rabbit polyclonal IgG) 2988840
      Anti-phospho-c-Met (Tyr1230/Tyr1234/Tyr1235) (Affinity purified Rabbit polyclonal IgG) - 2270325 2270325
      Anti-phospho-c-Met (Tyr1230/Tyr1234/Tyr1235) (Affinity purified Rabbit polyclonal IgG) - NG1936869 NG1936869
      Anti-phospho-c-Met (Tyr1230/Tyr1234/Tyr1235) - DAM1398295 DAM1398295
      Anti-phospho-c-Met (Tyr1230/Tyr1234/Tyr1235) - DAM1472187 DAM1472187

      References

      Reference overviewApplicationPub Med ID
      c-Met modulates RPE migratory response to laser-induced retinal injury.
      Kasaoka, M; Ma, J; Lashkari, K
      PloS one  7  e40771  2012

      Show Abstract
      Immunohistochemistry22808260 22808260
      A selective c-met inhibitor blocks an autocrine hepatocyte growth factor growth loop in ANBL-6 cells and prevents migration and adhesion of myeloma cells
      Hov, Håkon, et al
      Clin Cancer Res, 10:6686-94 (2004)  2004

      15475459 15475459
      Hepatocyte growth factor receptor tyrosine kinase met is a substrate of the receptor protein-tyrosine phosphatase DEP-1.
      Palka, Helena L, et al.
      J. Biol. Chem., 278: 5728-35 (2003)  2003

      Show Abstract
      12475979 12475979
      Mutations in the met oncogene unveil a "dual switch" mechanism controlling tyrosine kinase activity
      Chiara, Federica, et al
      J Biol Chem, 278:29352-8 (2003)  2003

      12746450 12746450
      Tyrosines1234-1235 are critical for activation of the tyrosine kinase encoded by the MET proto-oncogene (HGF receptor).
      Longati, P, et al.
      Oncogene, 9: 49-57 (1994)  1994

      Show Abstract
      8302603 8302603

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies