04-900 | Anti-phospho-Met (Tyr1234/1235) Antibody, clone NL8 | 04-900

04-900
100 µl  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-900
      Replaces05-900
      DescriptionAnti-phospho-Met (Tyr1234/1235) Antibody, clone NL8 | 04-900
      Alternate Names
      • HGF receptor
      • HGF/SF receptor
      • Met proto-oncogene tyrosine kinase
      • SF receptor
      • Scatter factor receptor
      • met proto-oncogene
      • met proto-oncogene (hepatocyte growth factor receptor)
      • oncogene MET
      Background InformationThe proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.
      References
      Product Information
      FormatPurified
      Control
      • RIPA lysates from A431 cells co-expressing Met/HGFR and HGF.
      PresentationCultured supernantant containing 0.05% sodium azide.
      Applications
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (…KE[pY][pY}SV…) in which pYpY corresponds to phosphorylated amino acids 1234 and 1235 of human Met/HGFR.
      EpitopepTyr1234/1235
      CloneNL8
      ConcentrationCultured supernantant containing 0.05% sodium azide.
      HostRabbit
      SpecificityRecognizes phosphorylated Met, Mr 140 kDa. Cross-reacts with phosphorylated EGFR.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various
      mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants
      encoding different isoforms have been found for this gene.
      Gene Symbol
      • AUTS9
      • EC 2.7.10.1
      • HGFR
      • OTTHUMP00000069168
      • RCCP2
      • c-Met
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      SUBUNIT STRUCTURE: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 By similarity. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN:The kinase domain is involved in SPSB1 binding.
      INVOLVEMENT IN DISEASE: Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. Defects in MET may be associated with gastric cancer.
      Defects in MET are a cause of hepatocellular carcinoma (HCC)
      Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance.
      Genetic variations in MET may be associated with susceptibility to autism type 9 (AUTS9) [MIM:611015]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family.
      Contains 3 IPT/TIG domains.
      Contains 1 protein kinase domain.
      Contains 1 Sema domain.

      Molecular Weight~140 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: A 1:10,000 dilution of this antibody detected phospho-Met in RIPA lysates from NIH-3T3 cells co-expressing Met/HGFR and HGF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies