05-774 | Anti-phospho-Neurofilament Antibody, medium chain, clone RMO55

05-774
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-774
      Replaces04-1061
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Neurofilament Antibody, medium chain, clone RMO55
      References
      Product Information
      FormatAscites
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect phospho-Neurofilament using this Anti-phospho-Neurofilament Antibody, medium chain, clone RMO55 validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenHPLC purified rat neurofilament isolated from rat spinal cords
      CloneRMO55
      HostMouse
      Specificityphospho-neurofilament, medium chain
      IsotypeIgM
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFM
      • NF-M
      • NFM
      • NEF3
      Modifications
      • Phosphorylation
      Purification MethodAscites
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
      SIZE: 916 amino acids; 102448 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.
      Molecular Weight~150kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on rat brain microsomal preparation (Catalog #12-144)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Neurofilament, medium chain, clone RMO55 (mouse ascites) - 2135168 2135168
      Anti-phospho-Neurofilament, medium chain, clone RMO55 - 26822 26822
      Anti-phospho-Neurofilament, medium chain, clone RMO55 -2654221 2654221
      Anti-phospho-Neurofilament, medium chain, clone RMO55 -2746248 2746248
      Anti-phospho-Neurofilament, medium chain, clone RMO55 -2806486 2806486

      References

      Reference overviewApplicationPub Med ID
      Erythropoietin produced by genetic-modified NIH/3T3 fibroblasts enhances the survival of degenerating neurons.
      Li, YC; Chen, SJ; Chien, CL
      Brain and behavior  5  e00356  2015

      Show Abstract
      26357589 26357589
      Requirement of heavy neurofilament subunit in the development of axons with large calibers
      Elder, G. A., et al
      J Cell Biol, 143:195-205 (1998)  1998

      Immunoblotting (Western)9763431 9763431
      Phosphate-dependent and independent neurofilament protein epitopes are expressed throughout the cell cycle in human medulloblastoma (D283 MED) cells.
      Trojanowski, J Q, et al.
      Am. J. Pathol., 135: 747-58 (1989)  1989

      Show Abstract
      2478022 2478022
      Monoclonal antibodies distinguish several differentially phosphorylated states of the two largest rat neurofilament subunits (NF-H and NF-M) and demonstrate their existence in the normal nervous system of adult rats.
      Lee, V M, et al.
      J. Neurosci., 7: 3474-88 (1987)  1987

      Show Abstract
      3119789 3119789
      Two-stage expression of neurofilament polypeptides during rat neurogenesis with early establishment of adult phosphorylation patterns.
      Carden, M J, et al.
      J. Neurosci., 7: 3489-504 (1987)  1987

      Show Abstract
      Immunohistochemistry (Tissue)3119790 3119790

      FAQ

      QuestionAnswer
      What is the concentration of this antibody?We are not able to quantitate ascites.

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies