05-663 | Anti-phospho-Nibrin/Nbs1 (Ser343) Antibody, clone 2G2E3

05-663
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-663
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Nibrin/Nbs1 (Ser343) Antibody, clone 2G2E3
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect phospho-Nibrin/Nbs1 (Ser343) with Anti-phospho-Nibrin/Nbs1 (Ser343) Antibody, clone 2G2E3 (Mouse Monoclonal Antibody), that has been demonstrated to work in IP.
      Key Applications
      • Immunoprecipitation
      Biological Information
      ImmunogenPeptide corresponding to amino acids 337-350 of human Nibrin/nbs1
      Clone2G2E3
      HostMouse
      SpecificityNibrin/nbs1 phosphorylated on serine 343
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • NBN
      • P95
      • MGC87362
      • Nibrin
      • FLJ10155
      • ATV
      • AT-V2
      • NBS
      • AT-V1
      • NBS1
      Modifications
      • Phosphorylation
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O60934 # Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability.


      SIZE: 754 amino acids; 84959 Da

      SUBUNIT: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A (By similarity). Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with TERF2 and with KPNA2.

      SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity).

      TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in testis.


      DOMAIN: SwissProt: O60934 The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage. & The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex. & The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.

      PTM: Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.

      DISEASE: SwissProt: O60934 # Defects in NBN are the cause of Nijmegen breakage syndrome (NBS) [MIM:251260]. NBS is an autosomal recessive syndrome characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies. & Defects in NBN are a cause of genetic susceptibility to breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. & Defects in NBN may be associated with aplastic anemia [MIM:609135]. Aplastic anemia is a disease of bone-marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. Most of the cases of aplastic anemia are idiopathic, some are familial and some are due to a viral infection or to exposure to chemicals and radiation. & Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).

      SIMILARITY: Contains 1 BRCT domain. & Contains 1 FHA domain.

      MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.
      Molecular Weight~100kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitating nibrin-containing immune complexes collected with anti-Nibrin (Catalog #07-317) from RIPA lysates of etoposide-treated, but not untreated, Jurkat cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Nibrin/Nbs1 (Ser343), clone 2G2E3 - 22247 22247
      Anti-phospho-Nibrin/nbs1 (Ser343), clone 2G2E3 - DAM1567743 DAM1567743
      Anti-phospho-Nibrin/nbs1 (Ser343), clone 2G2E3 -2578567 2578567

      References

      Reference overviewPub Med ID
      Sp1 facilitates DNA double-strand break repair through a nontranscriptional mechanism.
      Beishline, K; Kelly, CM; Olofsson, BA; Koduri, S; Emrich, J; Greenberg, RA; Azizkhan-Clifford, J
      Molecular and cellular biology  32  3790-9  2012

      Show Abstract
      22826432 22826432
      ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway.
      Lim, D S, et al.
      Nature, 404: 613-7 (2000)  2000

      Show Abstract
      10766245 10766245

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies