07-1830 | Anti-phospho-PDCD4 (Ser67) Antibody

07-1830
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1830
      DescriptionAnti-phospho-PDCD4 (Ser67) Antibody
      Alternate Names
      • Programmed cell death protein 4
      • Neoplastic transformation inhibitor protein
      • Nuclear antigen H731-like
      • Protein 197/15a
      Background InformationProgrammed cell death protein 4 (PDCD4) is widely studied for its role as a tumor suppressor. It inhibits the translation of RNA molecules indirectly, by binding to the eukaryotic initiation factor (elF) proteins that are involved in unwinding the secondary structure of RNA molecules, such as the p53 mRNA, before translation. PDCD4 tightly binds, via its C-terminal MA-3 domains, to elF4A and elF4G and consequently blocks protein synthesis and potential cell growth and proliferation, which suppress further development of transformed cells. PDCD4 is regulated by post-translational modification and proteosome degradation. It is phosphorylated on serine 67 by the S6K1 kinase and degraded by the SCF ubiquitin ligase. A number of studies have indicated that PDCD4 is downregulated in various human carcinomas.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Serum-starved and non-serum starved HeLa cell lysate.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-PDCD4 (Ser67) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-PDCD4 (Ser67) also known as Programmed cell death protein 4 & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: 0.2 µg/mL from a representative lot detected PDCD4 in an in-vitrokinase assay using recombinant GST-PDCD4 and S6K1 or S6K2 (Holcik, M., Department of Pediatrics, University of Ottawa, Ontario).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human PDCD4 phosphorylated at Ser67.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Xenopus, Chicken, Rat, Mouse, and Primate based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PDCD4
      • H731
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~56 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in serum-starved and non-serum starved HeLa cell lysate.

      Western Blot Analysis: 5 µg/mL of this antibody detected PDCD4 in 10 µg of serum-starved and non-serum starved HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies