04-1155 | Anti-phospho-PDGF Receptor β (Tyr857) Antibody, clone EP2136Y, rabbit monoclonal

04-1155
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1155
      DescriptionAnti-phospho-PDGF Receptor β (Tyr857) Antibody, clone EP2136Y, rabbit monoclonal
      Alternate Names
      • PDGF subunit B
      • PDGF, B chain
      • Platelet-derived growth factor B chain
      • Platelet-derived growth factor beta polypeptide
      • Platelet-derived growth factor, beta polypeptide (oncogene SIS)
      • platelet-derived growth factor 2
      • platelet-derived growth factor beta
      • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
      • platelet-derived growth factor, B chain
      Background InformationPDGF, which is a human platelet derived growth hormone, is a tyrosine kinase receptor. PDGF is involved in cell proliferation, migration, and survival. There are two isoforms of PDGF, PDGF receptor-α and PDGF receptor-β. PDGF receptor-β can only bind two specific PDGF isoforms, PDGF-β and PDGF-Δ. Tyr751 and Tyr740 are required for PDGFR-β mediated PI3 kinase activation. The second part of the tyrosine kinase domain in the PDGFβ receptor is phosphorylated at Tyr-857, and mutant receptors carrying phenylalanine at this position have reduced kinase activity. Tyr-857 has therefore been assigned a role in positive regulation of kinase activity.
      References
      Product Information
      FormatUnpurified
      Control
      • NIH/3T3 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationDetect phospho-PDGF Receptor β (Tyr857) using this Anti-phospho-PDGF Receptor β (Tyr857) Antibody, clone EP2136Y, Rabbit validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPhospho-specific peptide corresponding to residues surrounding Tyr857 of human PDGF-Receptor β.
      EpitopePhosphorylated Tyr857
      CloneEP2136Y
      HostRabbit
      SpecificityThis antibody detects PDGF-Receptor β phosphorylated at Tyr857.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
      Gene Symbol
      • Becaplermin
      • FLJ12858
      • PDGF-2
      • PDGF2
      • SIS
      • SSV
      • becaplermin
      • c-sis
      Modifications
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
      SUBUNIT STRUCTURE: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with XLKD1 By similarity.
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
      INVOLVEMENT IN DISEASE: A chromosomal aberration involving PDGFB is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with COLA1. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities.
      Pharmaceutical use Available under the name Regranex (Ortho-McNeil). Used to promote healing in diabetic neuropathic foot ulcers.
      MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor.
      SEQUENCE SIMILARITIES: Belongs to the PDGF/VEGF growth factor family.
      Molecular Weight~190 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on NIH/3T3 cell lysates.
      Western Blot Analysis: 1:500-1,000 dilution of this antibody was used to detect PDGF Receptor β in NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies