07-882 | Anti-phospho-PKCµ/PKD (Ser742) Antibody

07-882
100 µL  actual IgG concentration not measured; material titered to a working dilution of 1//1000 under standard western conditions
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-882
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-PKCµ/PKD (Ser742) Antibody
      Alternate Names
      • PRKD1
      • PKC-MU
      • PKCM
      • PKD
      • PRKCM
      • protein kinase C mu
      • protein kinase D1
      • PKCmu
      References
      Product Information
      FormatAffinity Purified
      Control
      • K562 cells treated with PMA or NIH3T3 cells treated with PDGF.
      Presentation100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS, pH 7.3 containing 1.0 mg/mL BSA and 0.05% sodium azide) in 50% glycerol.
      Applications
      ApplicationAnti-phospho-PKCμ/PKD (Ser742) Antibody detects level of phospho-PKCμ/PKD (Ser742) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse and rat PKCμ/PKD (Ser742).
      HostRabbit
      SpecificityRecognizes PKCμ/PKD phosphorylated on Ser742. Not expected to cross react with other corresponding phosphorylation sites on other PKC isoforms (α Thr497, βI &II Thr500, γ Thr514, δ Thr507, ε Thr566, ι Thr403, η Thr512, θ Thr538, or ζ Thr410 as) based on peptide competition experiments.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NotePredicted to cross-react Rat based on complete sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMembers of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM]
      Gene Symbol
      • PRKD1
      • PKCM
      • nPKC-mu
      • PKC-MU
      • PKC-mu
      • nPKC-D1
      • PKD
      • PKD1
      • PRKCM
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15139 # This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
      SIZE: 912 amino acids; 101888 Da
      SUBUNIT: Interacts via its N-terminal region with CENTA1.
      PTM: Autophosphorylated (By similarity).
      SIMILARITY: SwissProt: Q15139 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 PH domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Molecular Weight140 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Stable for 2 years at -20°C from date of shipment.
      Packaging Information
      Material Size100 µL
      Material Packageactual IgG concentration not measured; material titered to a working dilution of 1//1000 under standard western conditions
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PKC#236;/PKD (Ser742) (Affinity purified Rabbit polyclonal IgG) - DAM1412897 DAM1412897
      Anti-PKC#956;/PKD (Ser742) (Affinity purified Rabbit polyclonal IgG) - 2115521 2115521
      Anti-phospho-PKCmu;/PKD (Ser742) - 0605030674 0605030674
      Anti-phospho-PKCmu;/PKD (Ser742) - DAM1408797 DAM1408797
      Anti-phospho-PKCmu;/PKD (Ser742) - JBC1368781 JBC1368781

      References

      Reference overviewPub Med ID
      Differential PKC-dependent and -independent PKD activation by G protein α subunits of the Gq family: selective stimulation of PKD Ser⁷⁴⁸ autophosphorylation by Gαq.
      Waldron, RT; Innamorati, G; Torres-Marquez, ME; Sinnett-Smith, J; Rozengurt, E
      Cellular signalling  24  914-21  2012

      Show Abstract
      22227248 22227248
      Protein kinase D specifically mediates apoptosis signal-regulating kinase 1-JNK signaling induced by H2O2 but not tumor necrosis factor
      Zhang, Wei, et al
      J Biol Chem, 280:19036-44 (2005)  2005

      15755722 15755722
      Protein kinase D complexes with C-Jun N-terminal kinase via activation loop phosphorylation and phosphorylates the C-Jun N-terminus
      Hurd, Cliff, et al
      Oncogene, 21:2154-60 (2002)  2002

      11948398 11948398