06-821-I | Anti-phospho-PKCepsilon Antibody (Ser729)

100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWBRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number06-821-I
      DescriptionAnti-phospho-PKCepsilon Antibody (Ser729)
      Alternate Names
      • PKCepsilon
      • PKCe
      • Protein kinase C epsilon type
      • nPKC-epsilon
      Background InformationProtein Kinase C epsilon (PKC epsilon) is an ~87 kDa (migrates predominantly as 95 kDa in quiescient cells) member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC epsilon is an actin cytoskeleton regulating protein whose overexpression confers an oncogenic phenotype by affecting the ras signaling cascade. PKC epsilon is phosphorylated by PDK1 on threonine 566 and is then autophosphorylated on the turn motif threonine 710 and the hydrophobic motif serine 729. Catalytically inactive PKC epsilon is not phosphorylated on serine 729. Phosphorylation of serine 729 is mediated by mTOR-dependent pathways, plays an important role in regulating PKC epsilon subcellular localization, and is responsible for the appearance of the 92 kDa PKC epsilon species.
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-phospho-PKCepsilon Antibody (Ser729) is a highly specific rabbit polyclonal antibody, that targets PKC epsilon & has been tested in western blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting/Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot detected phospho PKCepsilon (Ser729) in 10 µg of NIH/3T3 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human PKC phosphorylated at Ser729.
      EpitopePhosphorylated Ser729
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody demonstrates cross-reactivity against the PKCbeta (Ser661) isoform, PKCdelta (Ser655) isoform, and possibly PKCalpha (Ser729), all of which contain the GF(pS)Y motif.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRKCE
      • PKC3
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~95 kDa and ~80 kDa observed. This protein has been observed at ~95 kDa and ~80 kDa (England, K. and Rumsby, M. G., et al. (2000). Biochem J. 352(Pt 1):19-26.).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting/Peptide Inhibition Analysis in C6 cell lysate.

      Western Blotting/Peptide Inhibition Analysis: A 1:1,000 dilution of this lot detected phospho PKCepsilon (Ser729) in 10 µg of C6 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-PKCepsilon (Ser729) 2476947
      Anti-phospho-PKCepsilon (Ser729) - Q2287280 Q2287280
      Anti-phospho-PKCepsilon (Ser729) -2555312 2555312
      Anti-phospho-PKCepsilon (Ser729) -2641709 2641709
      Anti-phospho-PKCepsilon (Ser729) -2687780 2687780
      Anti-phospho-PKCepsilon (Ser729) Polyclonal Antibody 2967252
      Anti-phospho-PKCepsilon (Ser729) Polyclonal Antibody 2910082
      Anti-phospho-PKCepsilon (Ser729)_2836857 2836857


      Reference overviewPub Med ID
      Uncovering potential downstream targets of oncogenic GRPR overexpression in prostate carcinomas harboring ETS rearrangements.
      Santos, J; Mesquita, D; Barros-Silva, JD; Jerónimo, C; Henrique, R; Morais, A; Paulo, P; Teixeira, MR
      Oncoscience  2  497-507  2015

      Show Abstract
      26097883 26097883

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      Life Science Research > Antibodies and Assays > Primary Antibodies