04-787 | Anti-phospho-PKD (Ser916) Antibody, clone MC29, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedMonoclonal Antibody
      Catalogue Number04-787
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-PKD (Ser916) Antibody, clone MC29, rabbit monoclonal
      Alternate Names
      • Protein kinase C mu type
      • Protein kinase D
      Background InformationAs members of the calcium-/calmodulin-depedent protein kinase superfamily, there are three known Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3. These serine/threonine kinases are involved in protein-protein and protein-carbohydrate adhesion and are also thought to be ion channel regulators. They are prominent downstream targets of protein kinase C and phospholipase D in various biological systems including mouse. PKDs are activated by various stimuli, including phorbol esters, G-protein-coupled receptors, reactive oxygen species, and diacylglycerol. PKDs regulate protein transport in the trans-Golgi network and are thought to play a role in muscle differentiation, cell motility, migration, and invasion. These isoforms are known to interact with each other. PKD2 is shown to be activated by phorbol esters, both in vivo and in vitro, and also by gastrin via the cholescystokinin/CCK(B) receptor in stably-transfected AGS-B cells. Furthermore, PKD2 is found to be active during the early phase of differentation of C2C12 cells into skeletal muscle cells. It is also reported to enhance TCR-induced NFAT activity in Jurkat T cells.
      Product Information
      • RIPA lysates from HEK293 cells
      PresentationCultured supernantant in 0.05% sodium azide
      ApplicationDetect phospho-PKD (Ser916) using this Anti-phospho-PKD (Ser916) Antibody, clone MC29 validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis:
      1:500-1:2000 of this lot detected PKD in RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate (PDBu) for 10 minutes at 50 ng/mL.
      Biological Information
      Immunogenpeptide containing RVpSIL in which pS corresponds to phospho-serine 916 of mouse PKD
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to cross-react with mouse based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMembers of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM]
      Gene Symbol
      • PRKD1
      • PKD
      • PKCM
      • nPKC-mu
      • PRKCM
      • PKC-mu
      • nPKC-D1
      • PKC-MU
      • PKD1
      • Phosphorylation
      UniProt Number
      Molecular Weight~105-110kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-PKD (Ser916), clone MC29 - 2161142 2161142
      Anti-phospho-PKD (Ser916), -2558447 2558447
      Anti-phospho-PKD (Ser916), -2576497 2576497
      Anti-phospho-PKD (Ser916), -2595526 2595526
      Anti-phospho-PKD (Ser916), clone MC29 - DAM1561699 DAM1561699


      Reference overviewPub Med ID
      Protein kinase D1 mediates stimulation of DNA synthesis and proliferation in intestinal epithelial IEC-18 cells and in mouse intestinal crypts.
      Sinnett-Smith, J; Rozengurt, N; Kui, R; Huang, C; Rozengurt, E
      The Journal of biological chemistry  286  511-20  2011

      Show Abstract Full Text Article
      21051537 21051537