05-1069 | Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2

05-1069
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1069
      DescriptionAnti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2
      Alternate Names
      • 40 kDa proline-rich AKT substrate
      • AKT1 substrate 1 (proline-rich)
      • proline-rich Akt substrate, 40 kDa
      Background InformationPRAS40 (Proline Rich Akt Substrate, 40kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1. It is thought that through its binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2 is validated for use in WB, IP, IC for the detection of phospho-PRAS40 (Thr246).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Biological Information
      ImmunogenSynthetic peptide corresponding to the amino acids surrounding and including phosphorylated Thr246 of human PRAS40.
      EpitopePhosphorylated Thr246
      Clone4H2.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityDetects PRAS40 when phosphorylated on Thr246.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and Mouse. Predicted to cross-react with rat and bovine based sequence homology of immunizing peptide.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]
      Gene Symbol
      • PRAS40
      • AKT1S1
      • Lobe
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
      SUBUNIT STRUCTURE: The phosphorylated form interacts with 14-3-3.
      SUBCELLULAR LOCATION: Cytoplasm - cytosol. Note= Found in the cytosolic fraction of the brain.
      TISUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).

      Molecular Weight40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on untreated and PDGF-treated NIH/3T3 cell lysate.

      Western Blot Analysis:
      1:1,000 dilution of this antibody was used to detect PRAS40 in PDGF-treated NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies