05-1056 | Anti-phospho-PTEN (Ser380) Antibody, clone 5.15.12, rabbit monoclonal

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, VrtWBRbCulture SupernatantMonoclonal Antibody
      Catalogue Number05-1056
      DescriptionAnti-phospho-PTEN (Ser380) Antibody, clone 5.15.12, rabbit monoclonal
      Alternate Names
      • MMAC1 phosphatase and tensin homolog deleted on chromosome
      • Mutated in multiple advanced cancers 1
      • phosphatase and tensin homolog
      Background InformationThe protein and lipid phosphatase PTEN (phosphatase and tensin homolog deleted on chromosome ten) has been suggested to play a role in tumorgenesis. PTEN is a tumor suppressor whose loss permits constitutive signaling through the PI3K pathway and is sufficient to cause cancer. In normal cells, FOXO1A and PTEN function together to restrain cell growth. PTEN metabolizes PI(3,4,5)P3, the product of PI3K phosphorylation of PI(4,5)P2 following receptor activation. In cells lacking PTEN, there is an elevated level of PI(3,4,5)P3, which acts as a potent second messenger promoting oncogenesis. Restoration of PTEN function is considered a novel approach to cancer therapy. Overexpression of PTEN inhibits cell migration, and expression of antisense PTEN mRNA enhances migration.
      Product Information
      FormatCulture Supernatant
      PresentationRabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.
      ApplicationThis Anti-phospho-PTEN (Ser380) Antibody, clone 5.15.12 is validated for use in WB for the detection of phospho-PTEN (Ser380).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids surrounding phosphorylated Ser380 of human PTEN.
      EpitopePhosphorylated Ser380 (C-terminus)
      SpecificityThis antibody detects PTEN when phosphorylated on Ser380.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Vertebrates
      Species Reactivity NoteHuman, Mouse, and Rat. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq]
      Gene Symbol
      • PTEN1
      • TEP1
      • MMAC1
      • MHAM
      • BZS
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.
      CATALYTIC ACTIVITY: Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 4,5-bisphosphate + phosphate.
      A phosphoprotein + H2O = a protein + phosphate.
      Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
      COFACTOR: Magnesium.
      SUBUNIT STRUCTURE: Monomer. The unphosphorylated form interacts with the second PDZ domain of AIP1 and with DLG1 and MAST2 in vitro. Interacts with MAGI2, MAGI3, MAST1 and MAST3, but neither with MAST4 nor with DLG5. Interaction with MAGI2 increases protein stability.
      SUBCELLULAR LOCATION: Cytoplasm. Ref.1
      TISSUE SPECIFICITY: Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
      INDUCTION: Down-regulated by transforming growth factor beta (TGF-beta). Ref.1
      DOMAIN: The C2 domain binds phospholipid membranes in vitro in a Ca2+-independent manner; this binding is important for its tumor suppressor function. Ref.14 Ref.21
      PTM: Phosphorylated in vitro by MAST1, MAST2 and MAST3. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding.
      INVOLVEMENT IN DISEASE: Mutations of PTEN are found in a large number of cancers.
      SEQUENCE SIMILARITIES: Contains 1 C2 tensin-type domain.
      Contains 1 phosphatase tensin-type domain.
      Molecular Weight55 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on NGF-treated PC12 cell lysate.

      Western Blot Analysis: 1:5,000 dilution of this antibody was used to detect PTEN phosphorylated on Ser380 in NGF-treated PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information