05-534 | Anti-phospho-Raf-1 (Ser338) Antibody

05-534
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-534
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Raf-1 (Ser338) Antibody
      References
      Product Information
      FormatPurified
      PresentationPBS, pH 7.4, with 0.09% sodium azide
      Applications
      ApplicationDetect phospho-Raf-1 (Ser338) using this Anti-phospho-Raf-1 (Ser338) Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to residues 334-347 of Raf-1; recognizes phosphorylated Raf-1 on Serine 338.
      HostRat
      SpecificityRaf-1 phosphorylated at Ser 338, irrespective of the phosphorylation state of Tyr341; does not bind to unactive Raf-1 or Raf-1 peptides phosphorylated only at Tyr341 or Ser339
      IsotypeIgG1
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
      Gene Symbol
      • RAF1
      • cRaf
      • Raf-1
      • c-Raf
      • RAF
      • CRAF
      • C-RAF
      Modifications
      • Phosphorylation
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
      COFACTOR: Binds 2 zinc ions per subunit.
      SIZE: 648 amino acids; 73052 Da
      SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
      Molecular Weight74kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on PDGF stimulated 3T3/NIH cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Raf-1 (Ser338) (rat monoclonal IgG1) - 2397075 2397075
      Anti-phospho-Raf-1 (Ser338) (rat monoclonal IgG1) - 2398810 2398810
      Anti-phospho-Raf-1 (Ser338) (rat monoclonal IgG1) 2925258
      Anti-phospho-Raf-1 (Ser338) (rat monoclonal IgG1) 2883131
      Anti-phospho-Raf-1 (Ser338) (rat monoclonal IgG1) 2884703
      Anti-phospho-Raf-1 (Ser338) (rat monoclonal IgG1) 2975899
      Anti-phospho-Raf-1 (Ser338) (rat monoclonal IgG1) - DAM1447119 DAM1447119
      Anti-phospho-Raf-1 (Ser338) - 19143 19143
      Anti-phospho-Raf-1 (Ser338) - 1977354 1977354
      Anti-phospho-Raf-1 (Ser338) - 19871 19871

      References

      Reference overviewPub Med ID
      Activation of protein kinase Calpha signaling prevents cytotoxicity and mutagenicity following lead acetate in CL3 human lung cancer cells.
      Chun-Yu Wang, Yun-Wei Lin, Jia-Ling Yang
      Toxicology  250  55-61  2008

      Show Abstract
      18590793 18590793
      Raf-1 kinase inhibitor protein: structure, function, regulation of cell signaling, and pivotal role in apoptosis.
      Odabaei, Golaun, et al.
      Adv. Cancer Res., 91: 169-200 (2004)  2004

      Show Abstract
      15327891 15327891
      Untying the regulation of the Raf-1 kinase.
      Dhillon, Amardeep S and Kolch, Walter
      Arch. Biochem. Biophys., 404: 3-9 (2002)  2002

      Show Abstract
      12127063 12127063
      Design and discovery of small molecules targeting raf-1 kinase.
      Lowinger, Timothy B, et al.
      Curr. Pharm. Des., 8: 2269-78 (2002)  2002

      Show Abstract
      12369855 12369855
      Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation.
      Mason, C S, et al.
      EMBO J., 18: 2137-48 (1999)  1999

      Show Abstract
      10205168 10205168

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      04-412 Anti-Raf-1 Antibody, rabbit monoclonal Show Pricing & Availability
      05-538 Anti-phospho-Raf-1 (Ser338) Antibody Show Pricing & Availability
      07-396 Anti-Raf-1 Antibody Show Pricing & Availability
      07-811 Anti-phospho-Raf1 (Ser259) Antibody Show Pricing & Availability
      07-815 Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies