07-815 | Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody

07-815
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-815
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Raf-1 (Tyr340/Tyr341) Antibody
      Alternate Names
      • CRAF
      • Raf1
      • c-Raf
      References
      Product Information
      FormatAffinity Purified
      Applications
      ApplicationDetect phospho-Raf-1 (Tyr340/Tyr341) using this Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesPredicted to cross-react with Mouse and Rat based on complete sequence homology.
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-Raf-1 (Tyr340/341).
      HostRabbit
      SpecificityRecognizes phospho-Raf-1 (Tyr340/341)
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to cross-react with Mouse and Rat based on complete sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
      Gene Symbol
      • RAF1
      • C-RAF
      • cRaf
      • Raf-1
      • c-Raf
      • CRAF
      • RAF
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
      COFACTOR: Binds 2 zinc ions per subunit.
      SIZE: 648 amino acids; 73052 Da
      SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
      Molecular Weight74 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Raf-1 (Tyr340/341) - 2013514 2013514
      Anti-phospho-Raf-1 (Tyr340/341) (Affinity purified Rabbit polyclonal IgG) - 2386934 2386934
      Anti-phospho-Raf-1 (Tyr340/341) (Affinity purified Rabbit polyclonal IgG) - 2159616 2159616
      Anti-phospho-Raf-1 (Tyr340/341) (Affinity purified Rabbit polyclonal IgG) - DAM1798675 DAM1798675
      Anti-phospho-Raf-1 (Tyr340/341) (Affinity purified Rabbit polyclonal IgG) - NG1926736 NG1926736
      Anti-phospho-Raf-1 (Tyr340/341) - 1982452 1982452
      Anti-phospho-Raf-1 (Tyr340/341) - DAM1411305 DAM1411305
      Anti-phospho-Raf-1 (Tyr340/341) - DAM1498501 DAM1498501
      Anti-phospho-Raf-1 (Tyr340/341) - DAM1717402 DAM1717402
      Anti-phospho-Raf-1 (Tyr340/341) - JBC1863390 JBC1863390

      References

      Reference overviewPub Med ID
      Dynamic changes in C-Raf phosphorylation and 14-3-3 protein binding in response to growth factor stimulation: differential roles of 14-3-3 protein binding sites
      Hekman, Mirko, et al
      J Biol Chem, 279:14074-86 (2004)  2004

      14688280 14688280
      Positive and negative regulation of Raf kinase activity and function by phosphorylation
      Chong, H, et al
      EMBO J, 20:3716-27 (2001)  2001

      11447113 11447113

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies