09-104 | Anti-phospho-Raptor (Ser722) Antibody

09-104
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Ca, Gt, Ch, EqDB, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-104
      DescriptionAnti-phospho-Raptor (Ser722) Antibody
      Alternate Names
      • P150 target of rapamycin (TOR)-scaffold protein
      • p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
      • regulatory associated protein of mTOR
      Background InformationRaptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Modified and non modified peptides of phosphorylated and non-phosphorylated Raptor protein
      PresentationPurified rabbit polyclonal in PBS with 0.09% sodium azide.
      Applications
      ApplicationAnti-phospho-Raptor (Ser722) Antibody detects level of phospho-Raptor (Ser722) & has been published & validated for use in DB & WB.
      Key Applications
      • Dot Blot
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Raptor phosphorylated at and around Ser722.
      EpitopeSer722
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Raptor phosphorylated at Ser722.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Canine
      • Goat
      • Chicken
      • Horse
      Species Reactivity NoteReacts with human. Predicted to react with mouse, rat, cow, dog, goat, chicken and horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • raptor
      • Raptor
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions.

      SIZE: 1335 amino acids; 149038 Da

      SUBUNIT: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non- phosphorylated form of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states with different stability.

      TISSUE SPECIFICITY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.

      SIMILARITY: Contains 7 WD repeats.

      MISCELLANEOUS: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient availability, and its potency for dissociation is increased under nutrient-rich conditions. This action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its intrinsic catalytic activity.
      Molecular Weight~ 150 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorylated Raptor protein.

      Dot Blot Analysis: 1 µg/mL of this antibody detected Raptor in modified and non modified peptides of phosphorylated and non-phosphorylated Raptor protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Raptor (Ser722) - 2433486 2433486
      Anti-phospho-Raptor (Ser722) - 1966945 1966945
      Anti-phospho-Raptor (Ser722) - 2197536 2197536
      Anti-phospho-Raptor (Ser722) - JBC1927878 JBC1927878
      Anti-phospho-Raptor (Ser722) - NG1746515 NG1746515
      Anti-phospho-Raptor (Ser722) - NG1858766 NG1858766
      Anti-phospho-Raptor (Ser722) - NRG1675704 NRG1675704
      Anti-phospho-Raptor (Ser722) -2594365 2594365
      Anti-phospho-Raptor (Ser722) -2605335 2605335
      Anti-phospho-Raptor (Ser722) Polyclonal Antibody 2975839

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies