09-106 | Anti-phospho-Raptor (Ser863) Antibody

09-106
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, XnDB, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-106
      DescriptionAnti-phospho-Raptor (Ser863) Antibody
      Alternate Names
      • P150 target of rapamycin (TOR)-scaffold protein
      • p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
      • regulatory associated protein of mTOR
      Background InformationRaptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Modified and non modified peptides of phosphorylated and non-phosphorlyated Raptor protein
      PresentationPurified rabbit serum in PBS with 0.10% sodium azide.
      Applications
      ApplicationAnti-phospho-Raptor (Ser863) Antibody is an antibody against phospho-Raptor (Ser863) for use in DB & WB.
      Key Applications
      • Dot Blot
      • Western Blotting
      Application NotesWestern Blot Analysis: 1;1000 dilution of this antibody has been shown to detect Raptor in 293 cell lysate expressing Raptor.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Raptor phosphorylated at Ser863.
      EpitopeSer863
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Raptor phosphorylated at Ser863.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Xenopus
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse, rat, and xenopus based on 100% sequence homology.
      Bovine and equine (90% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • raptor
      • Raptor
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Ref.1 Ref.2

      SUBUNIT STRUCTURE: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains FRAP1, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Forms a complex with FRAP1 under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation.

      TISSUE SPECIFICTY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.

      SEQUENCE SIMLARITIES: Belongs to the WD repeat RAPTOR family.

      Contains 7 WD repeats.
      Molecular Weight150 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated Raptor protein.

      Dot Blot Analysis: 1:1,000 dilution of this antibody detected Raptor in modified and non modified peptides of phosphorylated and non-phosphorlyated Raptor protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies