07-1331 | Anti-phospho-Rictor (Ser1219) Antibody

07-1331
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1331
      DescriptionAnti-phospho-Rictor (Ser1219) Antibody
      Alternate Names
      • Rapamycin-insensitive companion of mTOR
      • AVO3 homolog
      • hAVO3
      Background InformationRictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GµL. mTORC2 is activated by growth factors and other mitogenic signals.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rictor protein immunoprecipitated from 293T cells transfected with either wildtype Rictor or mutant Rictor (S1219A)
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-Rictor (Ser1219) Antibody is an antibody against phospho-Rictor (Ser1219) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Rictor phosphorylated at Ser1219.
      EpitopePhosphorylated Ser1219
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Rictor phosphorylated at Ser1219.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM].
      Gene Symbol
      • RICTOR
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

      SUBUNIT STRUCTURE: Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, LST8, PRR5, RICTOR and MAPKAP1. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to MTOR and PRR5 within the TORC2 complex. May interact with PRR5L.

      PTM: Phosphorylated by MTOR; when part of mTORC2.

      SEQUENCE SIMILARITIES: Belongs to the pianissimo family.

      SEQUENCE CAUTION: The sequence CAH18135.1 differs from that shown. Reason: Wrong choice of CDS.
      Molecular Weight ~225 kDa observed. The band at ~27 kDa is background reactivity to the light chain from the Rictor antibody.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Rictor protein immunoprecipitated from 293T cells transfected with either wildtype Rictor or mutant Rictor (S1219A).

      Western Blot Analysis: 1.25 µg/mL of this antibody detected Rictor on 10 µg of Rictor protein immunoprecipitated from 293T cells transfected with either wildtype Rictor or mutant Rictor (S1219A).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies