07-2273 | Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)

07-2273
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, CaWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2273
      DescriptionAnti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)
      Alternate Names
      • DCHT
      • Serine/threonine-protein kinase 39
      Background InformationAs members of the (sterile 20)-like (STE20) kinase family, the oxidative-stress-responsive kinase 1 (OSR1) and the SPS1-related proline/alanine-rich kinase (SPAK) serve as key enzymes in a signaling cascade, which mediate the activity of Na+/K+/2Cl- cotransporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which are 79% identical to each other at the sequence level, and are also highly conserved in orthologues of these enzymes in Caenorhabditis elegans, Drosophila, and Xenopus. The CCT domain reflects no similarity to sequences found in other proteins. This domain is known to recognize and interact with a unique peptide (Arg-Phe-Xaa-Val) motif, which is present in OSRT1- and SPAK-activating kinases (with-no-lysine-kinase 1 (WNK1 and WNK4) and also in STE20 kinase family substrates (NKCC1 and NKCC2). It is reported that phosphorylation of a Ser/Thr residue preceding the (Arg-Phe-Xaa-Val) motif results in steric clash, and subsequent dissociation from the CCT domain. Furthermore, patients suffering from Gordon syndrome carry mutated WNK1 and WNK4 genes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis phospho-SPAK antibody is validated for use in WB for the detection of the phospho-SPAK protein.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human SPAK and OSR1 phosphorylated at Ser375 and Ser325, respectively.
      EpitopeSPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      Species Reactivity NoteDemonstrated to react with Human and Mouse.
      Predicted to react with Rat and Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.
      Gene Symbol
      • STK39
      • SPAK
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May act as a mediator of stress-activated signals.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Nucleus when caspase-cleaved.
      Molecular Weight~60 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.

      Western Blot Anlaysis: 0.5 µg/mL of this antibody detected SPAK / OSR1 in 10 µg hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-SPAK (Ser373) / -2778850 2778850
      Anti-phospho-SPAK (Ser373) / -2794073 2794073
      Anti-phospho-SPAK (Ser373) / -2816629 2816629
      Anti-phospho-SPAK (Ser373) / phospho-OSR1 (Ser325) - 2118981 2118981
      Anti-phospho-SPAK (Ser373) / phospho-OSR1 (Ser325) - 2467156 2467156
      Anti-phospho-SPAK (Ser373) / phospho-OSR1 (Ser325) - 2022621 2022621
      Anti-phospho-SPAK (Ser373) / phospho-OSR1 (Ser325) - 2090493 2090493
      Anti-phospho-SPAK (Ser373) / phospho-OSR1 (Ser325) - 2211057 2211057
      Anti-phospho-SPAK (Ser373) / phospho-OSR1 (Ser325) - 2279269 2279269
      Anti-phospho-SPAK (Ser373) / phospho-OSR1 (Ser325) - 2343139 2343139

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies