04-478 | Anti-phospho-STAT1 (Ser 727) Antibody, clone 12C5

04-478
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-478
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-STAT1 (Ser 727) Antibody, clone 12C5
      References
      Product Information
      FormatPurified
      PresentationIn 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose, and 50% glycerol.
      Applications
      ApplicationAnti-phospho-STAT1 (Ser 727) Antibody, clone 12C5 is a Mouse Monoclonal Antibody for detection of phospho-STAT1 (Ser 727) also known as signal transducer & activator of transcription 1 & has been tested in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Serine727 on phosphorylated STAT1
      Clone12C5
      HostMouse
      SpecificityRecognizes phosphorylated domain of STAT1 on Serine727
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
      Gene Symbol
      • STAT1
      • ISGF-3
      • STAT91
      • DKFZp686B04100
      Modifications
      • Phosphorylation
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42224 # Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
      SIZE: 750 amino acids; 87335 Da
      SUBUNIT: Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. Interacts with NMI. Interacts with Sendai virus C', C, Y1 and Y2 proteins, Nipah virus P, V and W proteins, and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter (By similarity). Interaction with HCV core protein results in STAT1 degradation.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta.
      PTM: Phosphorylated on tyrosine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Serine phosphorylation is also required for maximal transcriptional activity in IFN-gamma transduction (lacking in beta form).
      DISEASE: SwissProt: P42224 # Defects in STAT1 are the cause of STAT1 deficiency [MIM:600555]. Patients generally suffer from mycobacterial or viral diseases. In the case of complete deficiency, patients can die of viral disease. & Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
      SIMILARITY: SwissProt: P42224 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain.
      Molecular Weight92 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-STAT1 (Ser 727), clone 12C5 - 0176S0102 0176S0102
      Anti-phospho-STAT1 (Ser 727), clone 12C5 -2522096 2522096

      References

      Reference overviewPub Med ID
      Prostacyclin receptor induces STAT1 and STAT3 phosphorylations in human erythroleukemia cells: a mechanism requiring PTX-insensitive G proteins, ERK and JNK
      Lo, Rico K H, et al
      Cell Signal, 18:307-17 (2006)  2006

      15979846 15979846
      VEGF receptors on chronic lymphocytic leukemia (CLL) B cells interact with STAT 1 and 3: implication for apoptosis resistance.
      Lee, Y K, et al.
      Leukemia, 19: 513-23 (2005)  2005

      Show Abstract
      15703780 15703780
      Differential effects of lipoprotein lipase on tumor necrosis factor-alpha and interferon-gamma-mediated gene expression in human endothelial cells
      Kota, Rama S, et al
      J Biol Chem, 280:31076-84 (2005)  2005

      15994321 15994321
      Roles of phosphatidylinositol 3-kinase in interferon-gamma-dependent phosphorylation of STAT1 on serine 727 and activation of gene expression
      Nguyen, H, et al
      J Biol Chem, 276:33361-8 (2001)  2001

      11438544 11438544

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies