07-714 | Anti-phospho-STAT1 (Ser727) Antibody

07-714
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-714
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-STAT1 (Ser727) Antibody
      Background InformationSTAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway.

      STAT 1, is activated by a number of different ligands, including IFNα, IFNγ, EGF, PDGF and IL6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
      References
      Product Information
      FormatSerum
      Control
      • PDGF treated NIH/3T3 cell lysate
      Applications
      ApplicationDetect phospho-STAT1 (Ser727) also known as signal transducer & activator of transcription 1 with Anti-phospho-STAT1 (Ser727) Antibody (rabbit polyclonal antibody), that has been demonstrated to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence (PM[pS]PE), in which [pS] corresponds to phospho-serine at residue 727 of human STAT1. The immunizing sequence is identical in mouse, rat, canine and chimpanzee.
      HostRabbit
      SpecificitySTAT1 phosphorylated on serine 727
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NotePredicted to cross-react with rat, canine and chimpanzee based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
      Gene Symbol
      • STAT1
      • ISGF-3
      • STAT91
      • DKFZp686B04100
      Modifications
      • Phosphorylation
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42224 # Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
      SIZE: 750 amino acids; 87335 Da
      SUBUNIT: Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. Interacts with NMI. Interacts with Sendai virus C', C, Y1 and Y2 proteins, Nipah virus P, V and W proteins, and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter (By similarity). Interaction with HCV core protein results in STAT1 degradation.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta.
      PTM: Phosphorylated on tyrosine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Serine phosphorylation is also required for maximal transcriptional activity in IFN-gamma transduction (lacking in beta form).
      DISEASE: SwissProt: P42224 # Defects in STAT1 are the cause of STAT1 deficiency [MIM:600555]. Patients generally suffer from mycobacterial or viral diseases. In the case of complete deficiency, patients can die of viral disease. & Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
      SIMILARITY: SwissProt: P42224 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain.
      Molecular Weight92kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-STAT1 (Ser727) - 30502 30502
      Anti-phospho-STAT1 (Ser727) -2642131 2642131
      Anti-phospho-STAT1 (Ser727) -2649136 2649136
      Anti-phospho-STAT1 (Ser727) -2673049 2673049
      Anti-phospho-STAT1 (Ser727) -2788172 2788172

      References

      Reference overviewPub Med ID
      Induction of STAT1 phosphorylation at serine 727 and expression of proinflammatory cytokines by porcine reproductive and respiratory syndrome virus.
      Yu, Y; Wang, R; Nan, Y; Zhang, L; Zhang, Y
      PloS one  8  e61967  2013

      Show Abstract
      23637938 23637938
      Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation.
      Wen, Z, et al.
      Cell, 82: 241-50 (1995)  1995

      Show Abstract
      7543024 7543024
      Requirement of serine phosphorylation for formation of STAT-promoter complexes.
      Zhang, X, et al.
      Science, 267: 1990-4 (1995)  1995

      Show Abstract
      7701321 7701321

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies