04-1059 | Anti-phospho-STAT3 (Tyr705) Antibody, clone EP2147Y, rabbit monoclonal

04-1059
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IH(P), ICC, IPRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1059
      DescriptionAnti-phospho-STAT3 (Tyr705) Antibody, clone EP2147Y, rabbit monoclonal
      Alternate Names
      • Acute-phase response factor
      • DNA-binding protein APRF
      • signal transducer and activator of transcription 3
      • signal transducer and activator of transcription 3
        (acute-phase response factor)
      Background InformationSTAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
      References
      Product Information
      FormatUnpurified
      Control
      • HeLa cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-phospho-STAT3 (Tyr705) Antibody, clone EP2147Y, Rabbit is an antibody against phospho-STAT3 (Tyr705) for use in WB, IH(P), IC & IP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected STAT3 in human colon adenocarcinoma tissue.
      Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected STAT3 in HeLa cells.
      Immunoprecipitation: A 1:40 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues surrounding phsophorylated Tyr705 of human STAT3.
      EpitopePhosphorylated Tyr705
      CloneEP2147Y
      HostRabbit
      SpecificityThis antibody recognizes STAT3 phsophorylated Tyr705.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encodingdistinct isoforms have been described.
      Gene Symbol
      • APRF
      • FLJ20882
      • HIES
      • MGC16063
      Modifications
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
      SUBUNIT STRUCTURE: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with NCOA1, PELP1, SOCS7 and STATIP1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts with IL31RA. Interacts with SIPAR. Interacts (via SH2 domain) with NLK By similarity. Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import By similarity. Interacts with TMF1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation.
      TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
      PTM: Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity.
      INVOLVEMENT IN DISEASE: Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
      MISCELLANEOUS: Involved in the gp130-mediated signaling pathway.
      SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
      Molecular WeightObserved MW: STAT3α (~84 kDa) and STAT3β (~76 kDa), depending on cell type.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on untreated and IFN-α-treated HeLa cell lysates.
      Western Blot Analysis: A 1:10,000-20,000 dilution of this antibody was used to detect STAT3 in IFNα-treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - 2121815 2121815
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - 2388827 2388827
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y 2470368
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - 2066719 2066719
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - 2204941 2204941
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - 2309342 2309342
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - 2519282 2519282
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - NG1698603 NG1698603
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - NG1753054 NG1753054
      Anti-phospho-STAT3 (Tyr705), clone EP2147Y, Rabbit Monoclonal - NG1820810 NG1820810

      References

      Reference overviewPub Med ID
      Reduced DICER1 elicits an interferon response in endometrial cancer cells.
      Chiappinelli, KB; Haynes, BC; Brent, MR; Goodfellow, PJ
      Molecular cancer research : MCR  10  316-25  2012

      Show Abstract
      22252463 22252463

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies