06-867 | Anti-phospho-STAT5A/B (Ser726/Ser731) Antibody

06-867
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-867
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-STAT5A/B (Ser726/Ser731) Antibody
      References
      Product Information
      FormatSerum
      Presentationrabbit antiserum in 30% glycerol and 0.05% sodium azide
      Applications
      ApplicationAnti-phospho-STAT5A/B (Ser726/Ser731) Antibody is a rabbit polyclonal antibody for detection of phospho-STAT5A/B (Ser726/Ser731) also known as signal transducer & activator of transcription 5A/B & has been tested in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide (DQAP[pS]PAVC) corresponding to amino acids 722-730 of human STAT5A or 727-735 of human STAT5B
      HostRabbit
      Specificityphosphorylated STAT5A (S726) and STAT5B (S731), does not recognize STAT5B containing a S731A mutation
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.
      Gene Symbol
      • STAT5A
      • MGF
      • STAT5
      Modifications
      • Phosphorylation
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42229 # Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
      SIZE: 794 amino acids; 90647 Da
      SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.
      PTM: Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL- 15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA- binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity).
      SIMILARITY: SwissProt: P42229 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain.
      Molecular Weight94kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitating STAT5A from RIPA lysates of Nb2-Sp cells that had been treated with prolactin; subsequent immunoblotting was performed using Anti-STAT5A (Catalog #06-968)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-STAT5A/B (S726/731) (rabbit antiserum) - 2468149 2468149
      Anti-phospho-STAT5A/B (S726/731) (rabbit antiserum) 2978903
      Anti-phospho-STAT5A/B (S726/731) (rabbit antiserum) 2927605
      Anti-phospho-STAT5A/B (S726/731) - DAM1745455 DAM1745455
      Anti-phospho-STAT5A/B (Ser726/Ser731) - 17444 17444
      Anti-phospho-STAT5A/B (Ser726/Ser731) - 23724 23724

      References

      Reference overviewPub Med ID
      Development of pure prolactin receptor antagonists.
      Bernichtein, S; Kayser, C; Dillner, K; Moulin, S; Kopchick, JJ; Martial, JA; Norstedt, G; Isaksson, O; Kelly, PA; Goffin, V
      The Journal of biological chemistry  278  35988-99  2003

      Show Abstract
      12824168 12824168
      Yin-yang 1 and glucocorticoid receptor participate in the Stat5-mediated growth hormone response of the serine protease inhibitor 2.1 gene.
      P L Bergad, H C Towle, S A Berry
      The Journal of biological chemistry  275  8114-20  2000

      Show Abstract
      10713133 10713133
      Differential control of the phosphorylation state of proline-juxtaposed serine residues Ser725 of Stat5a and Ser730 of Stat5b in prolactin-sensitive cells.
      Yamashita, H, et al.
      J. Biol. Chem., 273: 30218-24 (1998)  1998

      Show Abstract
      9804779 9804779
      Prolactin stimulates serine/tyrosine phosphorylation and formation of heterocomplexes of multiple Stat5 isoforms in Nb2 lymphocytes.
      Kirken, R A, et al.
      J. Biol. Chem., 272: 14098-103 (1997)  1997

      Show Abstract
      9162035 9162035
      Two discrete regions of interleukin-2 (IL2) receptor beta independently mediate IL2 activation of a PD98059/rapamycin/wortmannin-insensitive Stat5a/b serine kinase.
      Kirken, R A, et al.
      J. Biol. Chem., 272: 15459-65 (1997)  1997

      Show Abstract
      9182578 9182578

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies