04-886 | Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal

04-886
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M, B, ChpWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-886
      Replaces05-886
      DescriptionAnti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal
      Alternate Names
      • signal transducer and activator of transcription 5A
      • signal transducer and activator of transcription 5B
      • transcription factor STAT5B
      Background InformationThe STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • PDGF treated 3T3/NIH cells
      PresentationCultured supernatant containing 0.05% sodium azide.
      Applications
      ApplicationDetect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB.
      Key Applications
      • Western Blotting
      Applications Not Recommended
      • Immunoprecipitation
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B.
      EpitopepTyr694/699
      CloneA11W
      HostRabbit
      SpecificityPhospho-STAT5A/B (Tyr694/699).
      IsotypeIgG
      Species Reactivity
      • Rat
      • Human
      • Mouse
      • Bovine
      • Chimpanzee
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq]
      Gene Symbol
      • MGF
      • STAT5
      • STAT5
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

      SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 By similarity. Interacts with NCOA1 and SOCS7.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note= Translocated into the nucleus in response to phosphorylation.

      PTM: Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity By similarity.

      SIMILARITY: Belongs to the transcription factor STAT family.

      Contains 1 SH2 domain.

      Molecular Weight~95 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF.

      Western Blot Analysis:
      A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-STAT5A/B (Tyr694/699), clone A11W 2470805
      Anti-phospho-STAT5A/B (Tyr694/699), clone A11W - 2536514 2536514
      Anti-phospho-STAT5A/B (Tyr694/699), clone A11W -2576494 2576494
      Anti-phospho-STAT5A/B (Tyr694/699), clone A11W -2608738 2608738
      Anti-phospho-STAT5A/B (Tyr694/699), clone A11W -2714515 2714515

      References

      Reference overviewPub Med ID
      H2O2 production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling.
      Woolley, JF; Naughton, R; Stanicka, J; Gough, DR; Bhatt, L; Dickinson, BC; Chang, CJ; Cotter, TG
      PloS one  7  e34050  2012

      Show Abstract
      22807997 22807997
      Altered interleukin-12 responsiveness in Th1 and Th2 cells is associated with the differential activation of STAT5 and STAT1.
      Gollob, J A, et al.
      Blood, 91: 1341-54 (1998)  1998

      Show Abstract
      9454765 9454765

      Technical Info

      Title
      JAK/STAT Signaling Research Focus