05-1315 | Anti-phospho (Ser10) acetyl (Lys14) Histone H3 Antibody, clone 10H10.2

05-1315
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1315
      DescriptionAnti-phospho (Ser10) acetyl (Lys14) Histone H3 Antibody, clone 10H10.2
      Alternate Names
      • H3S10PK14Ac
      • Histone H3 (phospho S10, acetyl K14)
      • H3 histone family, member T
      • histone 3, H3
      • histone cluster 3, H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. The dual modification of histone H3 phosphorylated at serine 10 and acetylated at lysine 14 has been found at genomic sites with active transcription.
      References
      Product Information
      FormatPurified
      Control
      • Colcemid treated and untreated HeLa acid extract
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-phospho (Ser10) acetyl (Lys14) Histone H3 Antibody, clone 10H10.2 (Mouse Monoclonal Antibody) validated in WB to detect phospho (Ser10) acetyl (Lys14) Histone H3 also known as H3S10PK14Ac.
      Key Applications
      • Western Blotting
      Application NotesPeptide Block Analysis:
      To demonstrate specificity, 0.5 µg/ml of this antibody was tested against blocking and control peptides incubated with 10 µg of Colcemid treated HeLa acid extract.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to Histone H3 phosphorylated at Ser10 and acetylated at Lys14.
      EpitopeSer10 and Lys14
      Clone10H10.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Histone H3 phosphorylated at Ser10 and acetylated at Lys14.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Broad species cross-reactivity expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between cleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a
      replication-independent member of the histone H3 family. [provided by RefSeq].
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3t
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Modifications
      • Phosphorylation
      • Acetylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes.

      SUBCELLULAR LOCATION: Nucleus.

      DEVELOPMENTAL STAGE: Expressed throughout the cell cycle independently of DNA synthesis.

      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).

      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.

      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.

      Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.

      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, probably DAPK3. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase chromosomes.

      Ubiquitinated By similarity.

      SEQUENCE SIMILARITIES: Belongs to the histone H3 family.

      SEQUENCE CAUTION: The sequence CAH73371.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight ~ 15 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Colcemid treated and untreated HeLa acid extract.

      Western Blot Analysis: 0.001 µg/ml of this antibody detected Histone H3 on 10 µg of Colcemid treated and untreated HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho (Ser10) acetyl (Lys14) 2470960
      Anti-phospho (Ser10) acetyl (Lys14) -2748018 2748018
      Anti-phospho (Ser10) acetyl (Lys14) Histone H3, clone 10H10.2 - 2391059 2391059
      Anti-phospho (Ser10) acetyl (Lys14) Histone H3, clone 10H10.2 - 1967290 1967290
      Anti-phospho (Ser10) acetyl (Lys14) Histone H3, clone 10H10.2 - 2291708 2291708
      Anti-phospho (Ser10) acetyl (Lys14) Histone H3, clone 10H10.2 - 2310350 2310350
      Anti-phospho (Ser10) acetyl (Lys14) Histone H3, clone 10H10.2 - NRG1690166 NRG1690166

      References

      Reference overviewPub Med ID
      Inhibition of adenylyl cyclase type 5 prevents L-DOPA-induced dyskinesia in an animal model of Parkinson's disease.
      Park, HY; Kang, YM; Kang, Y; Park, TS; Ryu, YK; Hwang, JH; Kim, YH; Chung, BH; Nam, KH; Kim, MR; Lee, CH; Han, PL; Kim, KS
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  11744-53  2014

      Show Abstract
      25164669 25164669

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