06-702 | Anti-phospho-Smad1 (Ser463/465) Antibody

06-702
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, XnWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-702
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Smad1 (Ser463/465) Antibody
      Background InformationSmad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF-β receptor Serine/Threonine kinases and undergo serine phosphorylation in response to receptor activation. Following BMP (bone morphogenic protein) or TGF-β binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser 463 and Ser 465. Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1/Smad4 complex recruits DNA-binding proteins to activate specific gene transcription. BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription. In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways.
      References
      Product Information
      FormatPurified
      Control
      • BMP-4-treated HeLa or NIH/3T3 cells
      PresentationProtein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
      Applications
      ApplicationDetect phospho-Smad1 (Ser463/465) with Anti-phospho-Smad1 (Ser463/465) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to (CGGSPHNPISpSVpSGG) corresponding to amino acids 455-465 of human Smad1 protein
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes the dual serine phosphorylated Smad1 (Ser463/465).
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Xenopus
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
      Gene Symbol
      • SMAD1
      • MADR1
      • JV41
      • JV4-1
      • GARS
      • CMT2D
      • hSMAD1
      • HMN5
      • MADH1
      • BSP-1
      • BSP1
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15797 # Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).
      SIZE: 465 amino acids; 52260 Da
      SUBUNIT: Interacts with HGS and NANOG (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
      TISSUE SPECIFICITY: Ubiquitous. Highest expression seen in the heart and skeletal muscle.
      PTM: Phosphorylated on serine by BMP type 1 receptor kinase. & Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
      SIMILARITY: SwissProt: Q15797 ## Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain.
      Molecular Weight65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Smad1 (Ser463/465) - 17782 17782
      Anti-phospho-Smad1 (Ser463/465) - 21175 21175
      Anti-phospho-Smad1 (Ser463/465) - 24832 24832
      Anti-phospho-Smad1 (Ser463/465) -2782644 2782644
      Anti-phospho-Smad1 (Ser463/465) Polyclonal Antibody 2992395

      References

      Reference overviewApplicationPub Med ID
      ALK5 and ALK1 play antagonistic roles in transforming growth factor β-induced podosome formation in aortic endothelial cells.
      Curado, F; Spuul, P; Egaña, I; Rottiers, P; Daubon, T; Veillat, V; Duhamel, P; Leclercq, A; Gontier, E; Génot, E
      Molecular and cellular biology  34  4389-403  2014

      Show Abstract
      25266657 25266657
      Interaction between Smad1 and p97/VCP in rat testis and epididymis during the postnatal development.
      Sevil Cayli,Fikret Erdemir,Seda Ocakli,Bahadir Ungor,Hakan Kesici,Tamer Yener,Huseyin Aslan
      Reproductive sciences (Thousand Oaks, Calif.)  19  2012

      Show Abstract
      22051847 22051847
      Reduced endoglin activity limits cardiac fibrosis and improves survival in heart failure.
      Kapur, NK; Wilson, S; Yunis, AA; Qiao, X; Mackey, E; Paruchuri, V; Baker, C; Aronovitz, MJ; Karumanchi, SA; Letarte, M; Kass, DA; Mendelsohn, ME; Karas, RH
      Circulation  125  2728-38  2012

      Show Abstract
      22592898 22592898
      BMP2 promotes differentiation of nitrergic and catecholaminergic enteric neurons through a Smad1-dependent pathway.
      Anitha, Mallappa, et al.
      Am. J. Physiol. Gastrointest. Liver Physiol., 298: G375-83 (2010)  2010

      Show Abstract
      20007850 20007850
      Essential role of BMPs in FGF-induced secondary lens fiber differentiation.
      Bruce A Boswell, Paul A Overbeek, Linda S Musil
      Developmental biology  324  202-12  2008

      Show Abstract
      18848538 18848538
      Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells.
      Boswell, BA; Lein, PJ; Musil, LS
      Molecular biology of the cell  19  2631-41  2008

      Show Abstract Full Text Article
      18400943 18400943
      BMP signaling dynamics in embryonic orofacial tissue.
      Partha Mukhopadhyay,Cynthia L Webb,Dennis R Warner,Robert M Greene,M Michele Pisano
      Journal of cellular physiology  216  2008

      Show Abstract Full Text Article
      18446813 18446813
      Potentiation of astrogliogenesis by STAT3-mediated activation of bone morphogenetic protein-Smad signaling in neural stem cells.
      Fukuda, S; Abematsu, M; Mori, H; Yanagisawa, M; Kagawa, T; Nakashima, K; Yoshimura, A; Taga, T
      Molecular and cellular biology  27  4931-7  2007

      Show Abstract
      17452461 17452461
      Bone morphogenetic protein signaling and growth suppression in colon cancer.
      Beck, SE; Jung, BH; Fiorino, A; Gomez, J; Rosario, ED; Cabrera, BL; Huang, SC; Chow, JY; Carethers, JM
      American journal of physiology. Gastrointestinal and liver physiology  291  G135-45  2006

      Show Abstract
      16769811 16769811
      Bone morphogenetic protein 4 promotes pulmonary vascular remodeling in hypoxic pulmonary hypertension.
      Frank, DB; Abtahi, A; Yamaguchi, DJ; Manning, S; Shyr, Y; Pozzi, A; Baldwin, HS; Johnson, JE; de Caestecker, MP
      Circulation research  97  496-504  2005

      Show Abstract
      16100039 16100039

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies