04-953 | Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

04-953
100 µL  
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      Overview

      Replacement Information
      Replacement InformationCat. # 05-953. 05-953 will only be availabe on a custom basis.

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, ZebrafishWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-953
      Replaces05-953
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal
      Background InformationSMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationDetect phospho-Smad2 (Ser465/467) with Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S (Rabbit Monoclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2
      CloneA5S
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes phosphorylated Smad2. No Cross-reactivity with Smad3.
      IsotypeIgG
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Zebrafish
      Species Reactivity Note: Predicted to cross-react with X. laevis based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
      Gene Symbol
      • SMAD2
      • hMAD-2
      • MGC22139
      • JV18
      • MADH2
      • MGC34440
      • hSMAD2
      • MADR2
      • JV18-1
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15796 # Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
      SIZE: 467 amino acids; 52306 Da
      SUBUNIT: Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with SMAD3 and TRIM33. Interacts with SARA (SMAD anchor for receptor activation); may form trimers with the SMAD4 co-SMAD. Interacts with FOXH1, homeobox protein TGIF, PEBP2- alpha subunit, CREB-binding protein (CBP), p300, SKI, SNON and SMURF2. Interacts with AIP1 and HGS. Interacts with NEDD4L in response to TGF-beta (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
      TISSUE SPECIFICITY: Expressed at high levels in skeletal muscle, heart and placenta.
      PTM: Phosphorylated on one or several of Thr-220, Ser-245, Ser- 250, and Ser-255. In response to TGF-beta, phosphorylated on Ser- 465/467 by TGF-beta and activin type 1 receptor kinases. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. & In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation (By similarity).DISEASE:SwissProt: Q15796 # Defects in SMAD2 are found in sporadic cases of colorectal carcinoma.
      SIMILARITY: SwissProt: Q15796 ## Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain.
      Molecular Weight55-60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Smad2 (Ser465/467), -2549065 2549065
      Anti-phospho-Smad2 (Ser465/467), clone A5S - 2145085 2145085
      Anti-phospho-Smad2 (Ser465/467), clone A5S - 2398890 2398890
      Anti-phospho-Smad2 (Ser465/467), clone A5S - 1940983 1940983
      Anti-phospho-Smad2 (Ser465/467), clone A5S - 1952470 1952470
      Anti-phospho-Smad2 (Ser465/467), clone A5S - 2031814 2031814
      Anti-phospho-Smad2 (Ser465/467), clone A5S - 2061437 2061437
      Anti-phospho-Smad2 (Ser465/467), clone A5S - 2217722 2217722
      Anti-phospho-Smad2 (Ser465/467), clone A5S - 2272152 2272152
      Anti-phospho-Smad2 (Ser465/467), clone A5S - DAM1512950 DAM1512950

      References

      Reference overviewApplicationPub Med ID
      Activin A inhibits BMP-signaling by binding ACVR2A and ACVR2B.
      Olsen, OE; Wader, KF; Hella, H; Mylin, AK; Turesson, I; Nesthus, I; Waage, A; Sundan, A; Holien, T
      Cell communication and signaling : CCS  13  27  2015

      Show Abstract
      26047946 26047946
      Alk1 and Alk5 inhibition by Nrp1 controls vascular sprouting downstream of Notch.
      Aspalter, IM; Gordon, E; Dubrac, A; Ragab, A; Narloch, J; Vizán, P; Geudens, I; Collins, RT; Franco, CA; Abrahams, CL; Thurston, G; Fruttiger, M; Rosewell, I; Eichmann, A; Gerhardt, H
      Nature communications  6  7264  2015

      Show Abstract
      26081042 26081042
      The ventral to dorsal BMP activity gradient in the early zebrafish embryo is determined by graded expression of BMP ligands.
      Ramel, MC; Hill, CS
      Developmental biology  378  170-82  2013

      Show Abstract
      Western Blotting23499658 23499658
      Autocrine transforming growth factor-{beta}1 activation mediated by integrin {alpha}V{beta}3 regulates transcriptional expression of laminin-332 in Madin-Darby canine kidney epithelial cells.
      Moyano, JV; Greciano, PG; Buschmann, MM; Koch, M; Matlin, KS
      Molecular biology of the cell  21  3654-68  2010

      Show Abstract Full Text Article
      20844080 20844080
      Cathepsin K expression and activity in canine osteosarcoma.
      J M Schmit,H C Pondenis,A M Barger,L B Borst,L D Garrett,J M Wypij,Z L Neumann,T M Fan
      Journal of veterinary internal medicine / American College of Veterinary Internal Medicine  26  2001

      Show Abstract
      22171552 22171552

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies