07-1389 | Anti-phospho-Smad3 (Ser423/425) Antibody

07-1389
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostAntibody Type
      M, Mi, H, RWBRbPolyclonal Antibody
      Description
      Catalogue Number07-1389
      DescriptionAnti-phospho-Smad3 (Ser423/425) Antibody
      Alternate Names
      • MAD, mothers against decapentaplegic homolog 3
      • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
      • Mothers against DPP homolog 3
      • SMA- and MAD-related protein 3
      • SMAD 3
      • SMAD family member 3
      • SMAD, mothers against DPP homolog 3
      • SMAD, mothers against DPP homolog 3 (Drosophila)
      • mad homolog JV15-2
      • mad protein homolog
      • mothers against decapentaplegic homolog 3
      Background InformationMembers of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
      References
      Product Information
      PresentationUnpurified rabbit serum in buffer containing 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP homolog 3 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser423/425 of human Smad3.
      EpitopePhosphorylated Ser423/425
      HostRabbit
      SpecificityDetects phosphorylated Smad3 (Ser423 and Ser425).
      Species Reactivity
      • Mouse
      • Mink
      • Human
      • Rat
      Species Reactivity NoteMouse and Mink. Predicted to react with Human and Rat based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DKFZp586N0721
      • DKFZp686J10186
      • HSPC193
      • HsT17436
      • JV15-2
      • MADH3
      • MGC60396
      • Mad3
      • hMAD-3
      • hSMAD3
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD) By similarity.

      SUBUNIT STRUCTURE: Interacts with HGS. Interacts with NEDD4L in response to TGF-beta. Interacts with TTRAP By similarity. Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4. Interacts with JUN/FOS, vitamin D receptor, homeobox protein TGIF and TGIF2, PEBP2-alpha C subunit, CREB-binding protein (CBP), p300, SKI, SNON, ATF2, SMURF2, AIP1, DACH1 and TGFB1I1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Found in a complex with SMAD2 and TRIM33 upon addition of TGF-beta. Interacts with SMAD2 and TRIM33. Found in a complex with SMAD3, Ran and XPO4. Interacts with XPO4. Interacts with LBXCOR1 and CORL2.

      SUBCELLULAR LOCATION: CytoplasmBy similarity. NucleusBy similarity. Note= In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 By similarity.

      DOMAIN: The MH2 domain is sufficient to carry protein nuclear export.

      PTM: Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.

      INVOLVEMENT IN DISEASE: Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].

      SEQUENCE SIMILARITIES: Belongs to the dwarfin/SMAD family.

      Contains 1 MH1 (MAD homology 1) domain.

      Contains 1 MH2 (MAD homology 2) domain.

      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on untreated and UV-treated AKR-2B cell lysate.
      Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect Smad3 in UV-treated AKR-2B cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Smad3 (Ser423/425) - 2147136 2147136
      Anti-phospho-Smad3 (Ser423/425) - 1972817 1972817
      Anti-phospho-Smad3 (Ser423/425) - 1988586 1988586
      Anti-phospho-Smad3 (Ser423/425) - 2012974 2012974
      Anti-phospho-Smad3 (Ser423/425) - 2032735 2032735
      Anti-phospho-Smad3 (Ser423/425) - 2091769 2091769
      Anti-phospho-Smad3 (Ser423/425) - 2282194 2282194
      Anti-phospho-Smad3 (Ser423/425) - 2343145 2343145
      Anti-phospho-Smad3 (Ser423/425) - LV1662266 LV1662266
      Anti-phospho-Smad3 (Ser423/425) - NG1616373 NG1616373

      References

      Reference overviewApplicationPub Med ID
      Novel Function of Rev-erbα in Promoting Brown Adipogenesis.
      Nam, D; Chatterjee, S; Yin, H; Liu, R; Lee, J; Yechoor, VK; Ma, K
      Scientific reports  5  11239  2015

      Show Abstract
      26058812 26058812
      Eribulin mesilate suppresses experimental metastasis of breast cancer cells by reversing phenotype from epithelial-mesenchymal transition (EMT) to mesenchymal-epithelial transition (MET) states.
      Yoshida, T; Ozawa, Y; Kimura, T; Sato, Y; Kuznetsov, G; Xu, S; Uesugi, M; Agoulnik, S; Taylor, N; Funahashi, Y; Matsui, J
      British journal of cancer  110  1497-505  2014

      Show Abstract
      Western Blotting24569463 24569463
      Progenitor-like traits contribute to patient survival and prognosis in oligodendroglial tumors.
      Ng, FS; Toh, TB; Ting, EH; Koh, GR; Sandanaraj, E; Phong, M; Wong, SS; Leong, SH; Kon, OL; Tucker-Kellogg, G; Ng, WH; Ng, I; Tang, C; Ang, BT
      Clinical cancer research : an official journal of the American Association for Cancer Research  18  4122-35  2012

      Show Abstract
      22675171 22675171