05-677 | Anti-phospho-Src (Tyr416) Antibody, clone 9A6

05-677
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-677
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Src (Tyr416) Antibody, clone 9A6
      OverviewSpecific src isoform is unknown
      Alternate Names
      • proto-oncogene tyrosine-protein kinase SRC
      • protooncogene SRC
      • Rous sarcoma, tyrosine kinase pp60c-src
      • tyrosine-protein kinase SRC-1
      • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
      • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
      Background InformationSrc (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
      References
      Product Information
      FormatPurified
      Control
      • Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
      PresentationPurified mouse monoclonal IgG3 lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 mL H2O (15 min, RT). Lyophilized. Store at -20ºC.
      Applications
      ApplicationDetect phospho-Src (Tyr416) using this Anti-phospho-Src (Tyr416) Antibody, clone 9A6 validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation/Immunoblot Analysis: 4 μg/mL of a previous lot detected phospho-Src immunoprecipitated from 3T3 cell lysates with 10 μg of anti-Src (Catalog # 05-184), but not after phosphatase treatment with YOP.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src. Clone 9A6.
      Cloneclone 9A6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes phospho-Src, Mr 60kDa. The antibody does not crossreact with the nonphosphorylated form of Src or with unrelated phosphorylation sites. Predicted to recognize all Src family members phosphorylated at the tyrosine corresponding to Tyr416 of avian Src.
      IsotypeIgG3
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • SRC
      • c-src
      • p60-Src
      • SRC1
      • c-SRC
      • ASV
      • pp60c-src
      Modifications
      • Phosphorylation
      Purification MethodThiophilic adsoption and size exclusion chromatography
      UniProt Number
      UniProt SummarySIZE: 536 amino acids; 59835 Da
      SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
      SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by immunoprecipitating Src from 3T3 cell lysates with anti-Src (Catalog #05-184), but not after phosphatase treatment with YOP.

      Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Src (Tyr416), clone 9A6 - 2426513 2426513
      Anti-phospho-Src (Tyr416), -2519286 2519286
      Anti-phospho-Src (Tyr416), -2776859 2776859
      Anti-phospho-Src (Tyr416), -2820400 2820400
      Anti-phospho-Src (Tyr416), clone 9A6 - 0080S0606 0080S0606
      Anti-phospho-Src (Tyr416), clone 9A6 - 0080S0607 0080S0607
      Anti-phospho-Src (Tyr416), clone 9A6 - 1959685 1959685
      Anti-phospho-Src (Tyr416), clone 9A6 - 1997737 1997737
      Anti-phospho-Src (Tyr416), clone 9A6 - 2048766 2048766
      Anti-phospho-Src (Tyr416), clone 9A6 - 2069942 2069942

      References

      Reference overviewApplicationPub Med ID
      FAK inhibition abrogates the malignant phenotype in aggressive pediatric renal tumors.
      Megison, ML; Gillory, LA; Stewart, JE; Nabers, HC; Mrozcek-Musulman, E; Beierle, EA
      Molecular cancer research : MCR  12  514-26  2014

      Show Abstract
      24464916 24464916
      Individual Src-family tyrosine kinases direct the degradation or protection of the clock protein Timeless via differential ubiquitylation.
      O'Reilly, LP; Zhang, X; Smithgall, TE
      Cellular signalling  25  860-6  2013

      Show Abstract
      23266470 23266470
      Intercellular adhesion molecule-2 is involved in apical ectoplasmic specialization dynamics during spermatogenesis in the rat.
      Xiao, X; Cheng, CY; Mruk, DD
      The Journal of endocrinology  216  73-86  2013

      Show Abstract
      23097088 23097088
      Dasatinib inhibits the growth of prostate cancer in bone and provides additional protection from osteolysis.
      Koreckij, T; Nguyen, H; Brown, LG; Yu, EY; Vessella, RL; Corey, E
      British journal of cancer  101  263-8  2009

      Show Abstract Full Text Article
      19603032 19603032
      Epidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium.
      Lie, PP; Mruk, DD; Lee, WM; Cheng, CY
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  23  2555-67  2009

      Show Abstract
      19293393 19293393
      Mechanism of the vascular angiotensin II/alpha2-adrenoceptor interaction.
      Jackson, EK; Gao, L; Zhu, C
      The Journal of pharmacology and experimental therapeutics  314  1109-16  2005

      Show Abstract
      15901799 15901799
      Regulation of ectoplasmic specialization dynamics in the seminiferous epithelium by focal adhesion-associated proteins in testosterone-suppressed rat testes.
      Wong, CH; Xia, W; Lee, NP; Mruk, DD; Lee, WM; Cheng, CY
      Endocrinology  146  1192-204  2005

      Show Abstract
      Immunofluorescence15591141 15591141
      Alternative splicing modulates Disabled-1 (Dab1) function in the developing chick retina
      Katyal, S. and Godbout, R.
      Embo J, 23:1878-88 (2004)  2004

      Immunocytochemistry15057276 15057276
      Retinoblastoma protein functions as a molecular switch determining white versus brown adipocyte differentiation.
      Jacob B Hansen, Claus Jørgensen, Rasmus K Petersen, Philip Hallenborg, Rita De Matteis, Hans A Bøye, Natasa Petrovic, Sven Enerbäck, Jan Nedergaard, Saverio Cinti, Hein te Riele, Karsten Kristiansen
      Proceedings of the National Academy of Sciences of the United States of America  101  4112-7  2004

      Show Abstract Full Text Article
      15024128 15024128

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies