04-857 | Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal

04-857
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, MWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-857
      Replaces05-857
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
      Alternate Names
      • Proto-oncogene tyrosine-protein kinase Src
      • Proto-oncogene c-Src
      • pp60c-src
      • p60-Src
      Background InformationSrc (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • RIPA cell lysate
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationAnti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis:
      A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A).

      Immunoprecipitation:
      A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B).
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src
      Clone2N8
      HostRabbit
      Specificityphospho-c-Src (Tyr416)
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      • Mouse
      Species Reactivity NoteMouse. Broad species cross-reactivity expected based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • pp60c-src
      • ASV
      • c-SRC
      • SRC1
      • p60-Src
      • c-src
      • SRC
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummarySIZE: 536 amino acids; 59835 Da
      SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
      SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight60kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Src family (Tyr416), -2820671 2820671
      Anti-phospho-Src family (Tyr416), clone 2N8 - 2316552 2316552
      Anti-phospho-Src family (Tyr416), clone 2N8 - 2492808 2492808
      Anti-phospho-Src family (Tyr416), clone 2N8 - DAM1565967 DAM1565967
      Anti-phospho-Src family (Tyr416), clone 2N8 -2575901 2575901
      Anti-phospho-Src family (Tyr416), clone 2N8 Monoclonal Antibody 2956756

      References

      Reference overviewPub Med ID
      Structure and regulation of Src family kinases.
      Boggon, Titus J and Eck, Michael J
      Oncogene, 23: 7918-27 (2004)  2004

      Show Abstract
      15489910 15489910
      The interplay between Src family kinases and receptor tyrosine kinases.
      Bromann, Paul A, et al.
      Oncogene, 23: 7957-68 (2004)  2004

      Show Abstract
      15489913 15489913
      Molecular interdiction of Src-family kinase signaling in hematopoietic cells.
      Geahlen, Robert L, et al.
      Oncogene, 23: 8024-32 (2004)  2004

      Show Abstract
      15489920 15489920
      Src protein-tyrosine kinase structure and regulation.
      Roskoski, Robert
      Biochem. Biophys. Res. Commun., 324: 1155-64 (2004)  2004

      Show Abstract
      15504335 15504335
      Role of STATs as downstream signal transducers in Src family kinase-mediated tumorigenesis
      Silva, Corinne M
      Oncogene, 23:8017-23 (2004)  2004

      15489919 15489919

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