06-659 | Anti-phospho-c-Jun (Ser73) Antibody

06-659
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-659
      Replaces04-213
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-c-Jun (Ser73) Antibody
      Alternate Names
      • Activator protein 1
      • Jun activation domain binding protein
      • Proto-oncogene c-jun
      • V-jun avian sarcoma virus 17 oncogene homolog
      • enhancer-binding protein AP1
      • jun oncogene
      • v-jun sarcoma virus 17 oncogene homolog
      • v-jun sarcoma virus 17 oncogene homolog (avian)
      Background Informationc-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced
      signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Untreated and anisomycin-treated NIH/3T3 cell lysate.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-c-Jun (Ser73) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-c-Jun (Ser73) also known as Activator protein 1 & has been validated in ICC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot Analysis :
      A previous lot of this antibody detected 10 ng of phosphorylated gst-Jun, but did not detect 10 ng of nonphosphorylated Jun, as reported by an independent laboratory.

      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the human Phospho-C-Jun (Ser73) near the N-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody detects phospho-c-Jun. May also detect phospho-JunD in properly treated cells since the sequence homology is identical (13/13).
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine, Chicken, Zebrafish, Xenopus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
      Gene Symbol
      • JUN
      • p39
      • AP1
      • c-Jun
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Anisomycin treated NIH/3T3 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Phospho-C-Jun (Ser73) in 10 µg of Anisomycin treated NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2 to 8°C from date of shipment.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Phospho-C-Jun (Ser73) - 2500507 2500507
      Anti-Phospho-C-Jun (Ser73) -2524674 2524674
      Anti-Phospho-C-Jun (Ser73) -2552255 2552255
      Anti-Phospho-C-Jun (Ser73) -2566769 2566769
      Anti-Phospho-C-Jun (Ser73) -2596481 2596481
      Anti-Phospho-C-Jun (Ser73) -2649539 2649539
      Anti-Phospho-C-Jun (Ser73) -2700805 2700805
      Anti-Phospho-C-Jun (Ser73) Polyclonal Antibody 2985054
      Anti-beta Actin, clone EP1123Y, Rabbit Monoclonal - NG1726024 NG1726024
      Anti-phospho-c-Jun (Ser73) - 2117165 2117165

      References

      Reference overviewApplicationSpeciesPub Med ID
      Epinephrine Activation of the β2-Adrenoceptor Is Required for IL-13-Induced Mucin Production in Human Bronchial Epithelial Cells.
      Al-Sawalha, N; Pokkunuri, I; Omoluabi, O; Kim, H; Thanawala, VJ; Hernandez, A; Bond, RA; Knoll, BJ
      PloS one  10  e0132559  2015

      Show Abstract
      26161982 26161982
      RITA can induce cell death in p53-defective cells independently of p53 function via activation of JNK/SAPK and p38.
      Weilbacher, A; Gutekunst, M; Oren, M; Aulitzky, WE; van der Kuip, H
      Cell death & disease  5  e1318  2014

      Show Abstract
      Western Blotting25010984 25010984
      The detergent-soluble cytoplasmic pool of survivin suppresses anoikis and its expression is associated with metastatic disease of human colon cancer.
      Hori, M; Miki, T; Okamoto, M; Yazama, F; Konishi, H; Kaneko, H; Shimamoto, F; Ota, T; Temme, A; Tatsuka, M
      PloS one  8  e55710  2013

      Show Abstract
      Western Blotting23405201 23405201
      Immediate-early transcriptional response to angiotensin II in human adrenocortical cells.
      Thiel, G; Rössler, OG
      Endocrinology  152  4211-23  2011

      Show Abstract
      21914770 21914770
      Pregnenolone sulfate activates basic region leucine zipper transcription factors in insulinoma cells: role of voltage- gated Ca2+ channels and transient receptor potential melastatin 3 channels.
      Müller I, Rössler OG, Thiel G
      Molecular pharmacology  80  1179-89. Epub 2011 Sep 23.  2011

      Show Abstract
      21948387 21948387
      Thapsigargin induces expression of ATF3 in human keratinocytes involving Ca2+ ions and c-Jun N-terminal protein kinase.
      Spohn D, Rossler OG, Philipp SE, Raubuch M, Kitajima S, Griesemer D, Hoth M, Thiel G
      Mol Pharmacol  2010

      Show Abstract
      20713550 20713550
      c-Jun N-terminal kinase binding domain-dependent phosphorylation of mitogen-activated protein kinase kinase 4 and mitogen-activated protein kinase kinase 7 and balancing cross-talk between c-Jun N-terminal kinase and extracellular signal-regulated kinase pathways in cortical neurons.
      M Repici,L Mare,A Colombo,C Ploia,A Sclip,C Bonny,P Nicod,M Salmona,T Borsello
      Neuroscience  159  2009

      Show Abstract
      19135136 19135136
      Inhibition of apoptosome activation protects injured motor neurons from cell death.
      Kanungo, AK; Hao, Z; Elia, AJ; Mak, TW; Henderson, JT
      The Journal of biological chemistry  283  22105-12  2008

      Show Abstract
      Immunohistochemistry18550520 18550520
      Interplay and effects of temporal changes in the phosphorylation state of serine-302, -307, and -318 of insulin receptor substrate-1 on insulin action in skeletal muscle cells.
      Weigert, C; Kron, M; Kalbacher, H; Pohl, AK; Runge, H; Häring, HU; Schleicher, E; Lehmann, R
      Molecular endocrinology (Baltimore, Md.)  22  2729-40  2008

      Show Abstract
      18927238 18927238
      Transforming signals resulting from sustained activation of the PDGFbeta receptor in mortal human fibroblasts.
      Petti, Lisa M, et al.
      J. Cell. Sci., 121: 1172-82 (2008)  2008

      Show Abstract
      Human18349076 18349076

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies