04-213 | Anti-phospho-c-Jun (Ser73) Antibody, clone E107, rabbit monoclonal

04-213
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MDB, FC, ICC, IHC, IH(P), IP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-213
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-c-Jun (Ser73) Antibody, clone E107, rabbit monoclonal
      Alternate Names
      • Activator protein 1
      • Jun activation domain binding protein
      • Proto-oncogene c-jun
      • V-jun avian sarcoma virus 17 oncogene homolog
      • enhancer-binding protein AP1
      • jun oncogene
      • v-jun sarcoma virus 17 oncogene homolog
      Background InformationJun is a component of the transcription factor AP-1, and can constitute AP-1 activity either as a homodimer or as a heterodimer with Fos. By contrast with Fos, Jun is expressed constitutively, and can be activated by phosphorylation of serines 63 and/or 73. These sites are targets for the SAPK1/JNK family of protein kinases, and their phosphorylation states can be monitored using antibodies specific for the phospho-epitopes. In addition, Jun is phosphorylated by GSK3 on sites which inhibit Jun activity. Since GSK3 is inactivated by Akt, the Akt pathway results in Jun activation.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • NIH/3T3 cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-c-Jun (Ser73) using this Anti-phospho-c-Jun (Ser73) Antibody, clone E107 validated for use in DB, FC, IC, IH, IH(P), IP & WB.
      Key Applications
      • Dot Blot
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry: A 1:50 dilution of this antibody clone was was used in IHC.

      Immunocytochemistry: A 1:100-250 dilution of a previous lot was used in IC.

      Immunoprecipitation: A 1:80 dilution of a previous lot was used in IP.

      Flow Cytometry: A 1:20 dilution of a previous lot was used in FC.

      Dot Blot: A 1:500 dilution of a previous lot was used in DB.
      Biological Information
      ImmunogenKLH conjugated linear peptide corresponding to Phospho c-Jun.
      EpitopeSer73
      CloneE107
      HostRabbit
      SpecificityThis antibody recognizes phosphorylated c-Jun at and around Ser73.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteProven to react with human and mouse. Not expected to react with rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq]
      Gene Symbol
      • AP-1
      • AP1
      • OTTHUMP00000010036
      • c-Jun
      • p39
      Modifications
      • Phosphorylation
      UniProt Number
      Molecular Weight48 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on NIH/3T3 cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this lot was used to detect Phospho c-Jun NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies